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Literature summary for 2.7.7.65 extracted from
- Sol-gel immobilization of a thermophilic diguanylate cyclase for enzymatic production of cyclic-di-GMP (2010), J. Mol. Catal. B, 67, 98-103.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | immobilization of etDGC in sol-gel, a silica matrix that is known to encapsulate biological macromolecules via non-covalent entrapment, is described. The sol-gel matrix preserves the enzymatic activity of the enzyme, and thus, could be a viable method for DGC protein immobilization and c-di-GMP production | Thermotoga maritima |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Thermotoga maritima | cyclic di-3',5'-guanylate + diphosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diguanylate cyclase | - |
Thermotoga maritima |
| etDGC | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at | Thermotoga maritima |
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Release 2023.1 (January 2023)
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