Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as a His-tagged fusion protein is established and allows production of 2.5 mg of pure c-di-GMP per milligram of YdeH with standard biochemical lab equipment | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the reaction shows non-competitive product inhibition only at comparatively large c-di-GMP concentration | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | activity reduced about 20% in 50 mM LiCl | Escherichia coli | |
Na+ | 50 mM NaCl is used | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Escherichia coli | cyclic di-3',5'-guanylate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
diguanylate cyclase | - |
Escherichia coli |
YdeH | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |