Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.65 extracted from

  • De, N.; Navarro, M.V.; Raghavan, R.V.; Sondermann, H.
    Determinants for the activation and autoinhibition of the diguanylate cyclase response regulator WspR (2009), J. Mol. Biol., 393, 619-633.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.65

Activating Compound

Activating Compound Comment Organism Structure
BeF3-
-
 Pseudomonas syringae
additional information oligomerization-dependent activation of WspR, the stalks are key regulatory elements for the oligomerization, activation, and autoinhibition of WspR Pseudomonas syringae

Cloned(Commentary)

Cloned (Comment) Organism
the vectors pET21 and pProExHT are used Pseudomonas syringae

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of full-length WspR is determined at 2.8 A resolution, of WspGCN4-RGGDEF at 1.94 A resolution Pseudomonas syringae

Protein Variants

Protein Variants Comment Organism
WspGCN4-RGGDEF fusion protein, coiled-coil segment of GCN4 from Saccharomyces cerevisiae, C-terminal diguanylate cyclase domain with the characteristic GGDEF motif and the active site Pseudomonas syringae
WspRD70N mutant, point mutation at the predicted phosphorylation site in the REC domain Pseudomonas syringae
WspRGGDEF residues 172-347, C-terminal diguanylate cyclase domain with the characteristic GGDEF motif and the active site Pseudomonas syringae
WspRstalk-GGDEF residues 140-347, catalytic activity is modulated by the helical stalk motif, C-terminal diguanylate cyclase domain with the characteristic GGDEF motif and the active site Pseudomonas syringae

Inhibitors

Inhibitors Comment Organism Structure
cyclic di-3',5'-guanylate product inhibition Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00597
-
 GTP WspR full-length, cyclic di-3',5'-guanylate-free Pseudomonas syringae
0.01296
-
 GTP WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-free Pseudomonas syringae
0.01307
-
 GTP WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-bound Pseudomonas syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Pseudomonas syringae
-
 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Pseudomonas syringae

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA affinity chromatography, furthermore a Superdex 200 and a gel filtration column are used Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Pseudomonas syringae diphosphate + cyclic di-3',5'-guanylate
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Pseudomonas syringae

Synonyms

Synonyms Comment Organism
diguanylate cyclase
-
 Pseudomonas syringae
WspR
-
 Pseudomonas syringae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
 GTP WspR full-length, cyclic di-3',5'-guanylate-free Pseudomonas syringae
40.56
-
 GTP WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-bound Pseudomonas syringae
59.05
-
 GTP WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-free Pseudomonas syringae