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Literature summary for 2.7.7.65 extracted from

  • Lai, T.H.; Kumagai, Y.; Hyodo, M.; Hayakawa, Y.; Rikihisa, Y.
    The Anaplasma phagocytophilum PleC histidine kinase and PleD diguanylate cyclase two-component system and role of cyclic Di-GMP in host cell infection (2009), J. Bacteriol., 191, 693-700.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.65

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-33b+ for expression in Escherichia coli BL21DE3 cells Anaplasma phagocytophilum

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 theoretical Anaplasma phagocytophilum
52000
-
 native Anaplasma phagocytophilum PleD, determined by SDS-PAGE and Western Blot analysis Anaplasma phagocytophilum
56000
-
 recombinant PleD, determined by SDS-PAGE and Western Blot analysis Anaplasma phagocytophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Anaplasma phagocytophilum
-
 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Organism

Organism UniProt Comment Textmining
Anaplasma phagocytophilum Q2GKF8
-
-

Purification (Commentary)

Purification (Comment) Organism
using a His-Select cartridge Anaplasma phagocytophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Anaplasma phagocytophilum diphosphate + cyclic di-3',5'-guanylate
-
 ?

Synonyms

Synonyms Comment Organism
diguanylate cyclase
-
 Anaplasma phagocytophilum
PleD
-
 Anaplasma phagocytophilum