Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein | Leishmania major |
Crystallization (Comment) | Organism |
---|---|
crystal structures of a USP in the APO-, UTP-, and UDP-sugar-bound forms is presented. The overall structure of the enzyme exhibits a significant structural homology to other nucleotidyldiphosphate-glucose pyrophosphorylases. The broad substrate specificity is strongly correlated with the flexibility of the SB-loop and, in contrast to UDP-glucose pyrophosphorylase of Leishmania major, the spacious sugar binding cavity as well as the multiple conformations observed for the residues involved in sugar moiety binding | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania major | the enzyme preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | D3G6S4 | - |
- |
no activity in Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate | Leishmania major | ? | - |
? | |
additional information | the Leishmania major UDP-sugar pyrophosphorylase exhibits a broad substrate specificity in vitro | Leishmania major | ? | - |
? | |
UTP + alpha-D-galactose 1-phosphate | - |
Leishmania major | diphosphate + UDP-D-galactose | - |
? | |
UTP + alpha-D-galacturonate 1-phosphate | - |
Leishmania major | diphosphate + UDP-D-galacturonate | - |
? | |
UTP + alpha-D-glucose 1-phosphate | - |
Leishmania major | diphosphate + UDP-D-glucose | - |
r | |
UTP + alpha-L-arabinose 1-phosphate | - |
Leishmania major | diphosphate + UDP-L-arabinose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | overall structure of the enzyme, structure-function analysis, overview | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
UDP-sugar pyrophosphorylase | - |
Leishmania major |
USP | - |
Leishmania major |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme, likely involved in monosaccharide salvage, preferentially generates UDP-glucose and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate | Leishmania major |
additional information | active site conformations of apoenzyme and ligand-bound enzyme, substrate binding structures, structure-function analysis, modeling, overview | Leishmania major |