Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Trypanosoma cruzi |
gene TcSloppy, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Trypanosoma cruzi | |
0.026 | - |
UDP-D-glucose | pH 7.6, 37°C, recombinant enzyme | Trypanosoma cruzi | |
0.026 | - |
UDP-glucose | reverse reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
0.0284 | - |
UTP | pH 7.6, 37°C, recombinant enzyme | Trypanosoma cruzi | |
0.0284 | - |
UTP | forward reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
0.134 | - |
diphosphate | reverse reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
0.136 | - |
diphosphate | pH 7.6, 37°C, recombinant enzyme | Trypanosoma cruzi | |
0.177 | - |
alpha-D-glucose 1-phosphate | pH 7.6, 37°C, recombinant enzyme | Trypanosoma cruzi | |
0.177 | - |
D-glucose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
0.177 | - |
D-glucose 1-phosphate | forward reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
0.818 | - |
D-galactose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
1.76 | - |
D-glucose 1-phosphate | co-substrate: TTP, pH 7.6, 37°C | Trypanosoma cruzi | |
2.538 | - |
D-xylose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
2.54 | - |
TTP | co-substrate: glucose 1-phosphate, pH 7.6, 37°C | Trypanosoma cruzi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ required | Trypanosoma cruzi | |
Mg2+ | the enzyme requires bivalent ions, Mg2+ or Mn2+, for its activity | Trypanosoma cruzi | |
Mn2+ | Mg2+ or Mn2+ required | Trypanosoma cruzi | |
Mn2+ | the enzyme requires bivalent ions, Mg2+ or Mn2+, for its activity | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
SDS-PAGE | Trypanosoma cruzi |
67000 | - |
x * 67000, recombinant His-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma cruzi | the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars | ? | - |
? | |
TTP + alpha-D-glucose 1-phosphate | Trypanosoma cruzi | - |
diphosphate + TDP-D-glucose | - |
? | |
UTP + alpha-D-galactose 1-phosphate | Trypanosoma cruzi | - |
diphosphate + UDP-D-galactose | - |
? | |
UTP + alpha-D-glucose 1-phosphate | Trypanosoma cruzi | - |
diphosphate + UDP-D-glucose | - |
r | |
UTP + alpha-D-glucuronate 1-phosphate | Trypanosoma cruzi | - |
diphosphate + UDP-D-glucuronate | - |
? | |
UTP + alpha-D-xylose 1-phosphate | Trypanosoma cruzi | - |
diphosphate + UDP-D-xylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Trypanosoma cruzi | D3YBH2 | gene Sloppy | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration | Trypanosoma cruzi |
using Ni-NTA chromatography | Trypanosoma cruzi |
Storage Stability | Organism |
---|---|
-80°C, flash-frozen in liquid nitrogen, several months | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose 1-phosphate + UTP | - |
Trypanosoma cruzi | UDP-galactose + diphosphate | - |
r | |
D-glucose 1-phosphate + TTP | - |
Trypanosoma cruzi | TTP-glucose + diphosphate | - |
r | |
D-glucose 1-phosphate + UTP | - |
Trypanosoma cruzi | UDP-glucose + diphosphate | - |
r | |
D-xylose 1-phosphate + UTP | - |
Trypanosoma cruzi | UDP-xylose + diphosphate | - |
r | |
diphosphate + UDP-D-glucose | - |
Trypanosoma cruzi | UTP + alpha-D-glucose 1-phosphate | - |
r | |
additional information | the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars | Trypanosoma cruzi | ? | - |
? | |
TTP + alpha-D-glucose 1-phosphate | - |
Trypanosoma cruzi | diphosphate + TDP-D-glucose | - |
? | |
UTP + alpha-D-galactose 1-phosphate | - |
Trypanosoma cruzi | diphosphate + UDP-D-galactose | - |
? | |
UTP + alpha-D-glucose 1-phosphate | - |
Trypanosoma cruzi | diphosphate + UDP-D-glucose | - |
r | |
UTP + alpha-D-glucuronate 1-phosphate | - |
Trypanosoma cruzi | diphosphate + UDP-D-glucuronate | - |
? | |
UTP + alpha-D-xylose 1-phosphate | - |
Trypanosoma cruzi | diphosphate + UDP-D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, recombinant His-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
More | real-time NMR analysis of TcSloppy, overview | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
TcSloppy | - |
Trypanosoma cruzi |
UDP-sugar pyrophosphorylase | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 42 | - |
Trypanosoma cruzi |
37 | - |
assay at | Trypanosoma cruzi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 42 | highly active between | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
TTP | co-substrate: glucose 1-phosphate, pH 7.6, 37°C | Trypanosoma cruzi | |
0.12 | - |
D-glucose 1-phosphate | co-substrate: TTP, pH 7.6, 37°C | Trypanosoma cruzi | |
8.6 | - |
D-xylose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
26 | - |
D-glucose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
35.2 | - |
D-galactose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
maximum activity in phosphate buffer | Trypanosoma cruzi |
6.5 | 8 | - |
Trypanosoma cruzi |
7.6 | - |
assay at | Trypanosoma cruzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | highly active between | Trypanosoma cruzi |
7.5 | 7.8 | maximum activity in Tris buffer | Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis | Trypanosoma cruzi |
physiological function | the parasite enzyme with such broad substrate specificities suggests an alternative pathway that might play an essential role for nucleotide-sugar biosynthesis and for the regulation of the NDP-sugar pool in the parasite | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0334 | - |
TTP | co-substrate: glucose 1-phosphate, pH 7.6, 37°C | Trypanosoma cruzi | |
0.068 | - |
D-glucose 1-phosphate | co-substrate: TTP, pH 7.6, 37°C | Trypanosoma cruzi | |
34 | - |
D-xylose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
40 | - |
D-galactose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
150 | - |
D-glucose 1-phosphate | co-substrate: UTP, pH 7.6, 37°C | Trypanosoma cruzi | |
150 | - |
D-glucose 1-phosphate | forward reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
220 | - |
diphosphate | reverse reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
920 | - |
UTP | forward reaction, pH 7.6, 37°C | Trypanosoma cruzi | |
1150 | - |
UDP-glucose | reverse reaction, pH 7.6, 37°C | Trypanosoma cruzi |