Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1-phosphate | 2 mM, 78% activation | Thermus caldophilus | |
galactose 6-phosphate | 2 mM, 70% activation | Thermus caldophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
UTP | pH 8.0, 73°C | Thermus caldophilus | |
0.6 | - |
N-acetylglucosamine 1-phosphate | pH 8.0, 73°C | Thermus caldophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent metal required. Mn2+ (3 mM) is 63% as effective as Mg2+ (3 mM) | Thermus caldophilus | |
Mg2+ | divalent metal required. Order of decreasing effectiveness: Mg2+ > Mn2+ > Co2+. Optimal concentration of Mg2+ is 5 mM | Thermus caldophilus | |
Mn2+ | divalent metal required. Mn2+ (3 mM) is 87% as effective as Mg2+ (3 mM) | Thermus caldophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE | Thermus caldophilus |
45000 | - |
gel filtration | Thermus caldophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus caldophilus | - |
GK24 | - |
Thermus caldophilus GK24 | - |
GK24 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus caldophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + D-galactose 1-phosphate | 3% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus | diphosphate + UDP-galactose | - |
? | |
UTP + D-galactose 1-phosphate | 3% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus GK24 | diphosphate + UDP-galactose | - |
? | |
UTP + D-glucose 1-phosphate | 159% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus | diphosphate + UDP-glucose | - |
? | |
UTP + D-glucose 1-phosphate | 159% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus GK24 | diphosphate + UDP-glucose | - |
? | |
UTP + D-mannose 1-phosphate | 5% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus | diphosphate + UDP-mannose | - |
? | |
UTP + D-mannose 1-phosphate | 5% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus GK24 | diphosphate + UDP-mannose | - |
? | |
UTP + D-xylose 1-phosphate | 44% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus | diphosphate + UDP-alpha-D-xylopyranose | - |
? | |
UTP + D-xylose 1-phosphate | 44% of the activity with N-acetylglucosamine 1-phosphate | Thermus caldophilus GK24 | diphosphate + UDP-alpha-D-xylopyranose | - |
? | |
UTP + N-acetylglucosamine 1-phosphate | - |
Thermus caldophilus | diphosphate + UDP-N-acetylglucosamine | - |
? | |
UTP + N-acetylglucosamine 1-phosphate | - |
Thermus caldophilus GK24 | diphosphate + UDP-N-acetylglucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Thermus caldophilus |
Synonyms | Comment | Organism |
---|---|---|
UDP-sugar pyrophosphorylase | - |
Thermus caldophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
86 | - |
- |
Thermus caldophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 95 | 65°C: about 60% of maximal activity, 95°C: 90% of maximal activity | Thermus caldophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 30 min | Thermus caldophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
N-acetylglucosamine 1-phosphate | pH 8.0, 73°C | Thermus caldophilus | |
0.4 | - |
UTP | pH 8.0, 73°C | Thermus caldophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
in presence of Mg2+ | Thermus caldophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 90% of maximal activity, pH 8.0: about 75% of maximal activity | Thermus caldophilus |