Literature summary for 2.7.7.64 extracted from

Ohashi, T.; Cramer, N.; Ishimizu, T.; Hase, S.
Preparation of UDP-galacturonic acid using UDP-sugar pyrophosphorylase (2006), Anal. Biochem., 352, 182-187.

Search for more literature for 2.7.7.64

Application

Application	Comment	Organism
synthesis	the enzyme is useful for the highly efficient production of UDP-galacturonic acid for studies on pectin biosynthesis	Pisum sativum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pisum sativum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.7	-	diphosphate	pH 7.0, 35°C	Pisum sativum
1.15	-	D-galacturonic acid 1-phosphate	pH 7.0, 35°C	Pisum sativum
1.26	-	UDP-galacturonic acid	pH 7.0, 35°C	Pisum sativum
2.27	-	UTP	pH 7.0, 35°C	Pisum sativum

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UTP + D-galacturonic acid 1-phosphate	equilibrium constant is 0.24	Pisum sativum	diphosphate + UDP-galacturonic acid	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Pisum sativum

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	pH 5.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity	Pisum sativum

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Release 2023.1 (January 2023)