Any feedback?
Literature summary for 2.7.7.62 extracted from
- Biochemical characterization of the GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the hyperthermophilic archaeon Methanocaldococcus jannaschii (2009), Biochemistry, 48, 5882-5889.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pT7-7 for expression in Escherichia coli BL21-CodonPlus DE3 cells | Methanocaldococcus jannaschii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
GTP | apparent KM | Methanocaldococcus jannaschii |  |
| 0.0184 | - |
adenosylcobinamide phosphate | apparent KM | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21600 | - |
monomer, determined by gel filtation and SDS-PAGE | Methanocaldococcus jannaschii |
| 43000 | - |
dimer, determined by gel filtation | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + adenosylcobinamide phosphate | Methanocaldococcus jannaschii | - |
diphosphate + adenosylcobinamide-GDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | Q58517 | MJ1117 open reading frame | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| in two steps by hydrophobic interaction and ion-exchange chromatography | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii | diphosphate + adenosylcobinamide-GDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 21600 | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CobY | - |
Methanocaldococcus jannaschii |
| GTP:adenosylcobinamide-phosphate guanylyltransferase | - |
Methanocaldococcus jannaschii |
| MjCobY | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
kinetic assay | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
MjCobY denaturates at 80°C with a concomitant loss of activity | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
GTP | - |
Methanocaldococcus jannaschii | |
| 4.7 | - |
adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
