Cloned (Comment) | Organism |
---|---|
in pET-16b (pCLK740) for expression with N-terminal deca-His-tag in Salmonella enterica serovar Typhimurium LT2, strain JE8407 lacking endogenous CobU and YcfN | Escherichia coli |
General Stability | Organism |
---|---|
no soluble N-terminal His-tagged protein detectable after immobilized metal affinity chromatography from over-expressing Salmonella enterica serovar Typhimurium LT2 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica | - |
serovar typhimurium LT2 | - |
Purification (Comment) | Organism |
---|---|
tandem hydrophobic interaction chromatography on two HiTrap Phenyl FastFlow columns, dialysation, and dye ligand chromatography on HiTrap Blue HP column (elution with 2 M NaCl) | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide-GDP + diphosphate | 2 mM ATP, 5 mM MgCl2, 16 h, pH 8, 37°C | Salmonella enterica | GTP + adenosylcobinamide phosphate | reaction stop by 25 mM KCN and 10 min heating at 80°C, analysis by mass spectrometry | ? | |
adenosylcobinamide-GDP + diphosphate | over-expressing cell extracts, 2 mM ATP, 5 mM MgCl2, 16 h, pH 8, 37°C | Escherichia coli | GTP + adenosylcobinamide phosphate | reaction stop by 25 mM KCN and 10 min heating at 80°C, analysis by mass spectrometry | ? |
Synonyms | Comment | Organism |
---|---|---|
CobU | - |
Salmonella enterica |
GTP: adenosylcobinamide-phosphate guanylyltransferase | GTP: AdoCbi-P guanylyltransferase | Salmonella enterica |
NTP: adenosylcobinamide kinase | NTP: AdoCbi kinase | Salmonella enterica |
thiamine kinase | - |
Escherichia coli |
YcfN | - |
Escherichia coli |