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Literature summary for 2.7.7.60 extracted from

  • Brault, J.P.; Friesen, J.A.
    Characterization of cytidylyltransferase enzyme activity through high performance liquid chromatography (2016), Anal. Biochem., 510, 26-32.
    View publication on PubMed

Application

Application Comment Organism
analysis CTP:phosphocholine cytidylyltransferase enzyme assay that employs separation of non-radioactive 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from CTP. The assay can be applied to glycerol 3-phosphate cytidylyltransferase and CTP:2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase (CMS), members of the cytidylyltransferase family that produce CDP-glycerol and CDP-methylerythritol, respectively Listeria monocytogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 2-C-methyl-D-erythritol 4-phosphate Listeria monocytogenes
-
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
Listeria monocytogenes diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?
additional information using an assay method for determination of the catalytic activity measuring the production of radiolabeled 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from 14C-labeled 2-C-methyl-D-erythritol 4-phosphate, and separation of non-radioactive 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from CTP by C18 reverse phase HPLC with a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4 Listeria monocytogenes ?
-
?

Synonyms

Synonyms Comment Organism
CMS
-
Listeria monocytogenes
CTP:2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase
-
Listeria monocytogenes
cytidylyltransferase
-
Listeria monocytogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Listeria monocytogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Listeria monocytogenes

General Information

General Information Comment Organism
evolution the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids Listeria monocytogenes