Application | Comment | Organism |
---|---|---|
analysis | CTP:phosphocholine cytidylyltransferase enzyme assay that employs separation of non-radioactive 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from CTP. The assay can be applied to glycerol 3-phosphate cytidylyltransferase and CTP:2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase (CMS), members of the cytidylyltransferase family that produce CDP-glycerol and CDP-methylerythritol, respectively | Listeria monocytogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2-C-methyl-D-erythritol 4-phosphate | Listeria monocytogenes | - |
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2-C-methyl-D-erythritol 4-phosphate | - |
Listeria monocytogenes | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
additional information | using an assay method for determination of the catalytic activity measuring the production of radiolabeled 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from 14C-labeled 2-C-methyl-D-erythritol 4-phosphate, and separation of non-radioactive 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from CTP by C18 reverse phase HPLC with a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4 | Listeria monocytogenes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMS | - |
Listeria monocytogenes |
CTP:2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase | - |
Listeria monocytogenes |
cytidylyltransferase | - |
Listeria monocytogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Listeria monocytogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Listeria monocytogenes |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids | Listeria monocytogenes |