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Literature summary for 2.7.7.60 extracted from

  • Rohdich, F.; Wungsintaweekul, J.; Fellermeier, M.; Sagner, S.; Herz, S.; Kis, K.; Eisenreich, W.; Bacher, A.; Zenk, M.H.
    Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol (1999), Proc. Natl. Acad. Sci. USA, 96, 11758-11763.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.60

Cloned(Commentary)

Cloned (Comment) Organism
hyperexpression in Escherichia coli XL1-Blue Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00314
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli
0.131
-
 CTP
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Arabidopsis thaliana 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ catalytically active with Escherichia coli
Mg2+
-
 Arabidopsis thaliana
Mg2+ catalytically active with Escherichia coli
Mn2+ catalytically active with Escherichia coli
additional information Cu2+, Ni2+, Ca2+, Fe2+ or Zn2+ do not serve as cofactor Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Escherichia coli
50000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 2-C-methyl-D-erythritol-4-phosphate Arabidopsis thaliana
-
 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli
-
 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis ?
CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli DH5-alpha
-
 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Escherichia coli
-
 DH5alpha
-
Escherichia coli DH5-alpha
-
 DH5alpha
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose Q FF column, Phenyl Sepharose, Cibacron blue 3GA column, Red Sepharose CL-6B column, Source 15 Q column Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23
-
 pure protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 2-C-methyl-D-erythritol-4-phosphate
-
 Arabidopsis thaliana diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis ?
CTP + 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli DH5-alpha diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Escherichia coli
dimer homodimer Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana
8
-
 assay at Escherichia coli
8.3
-
-
 Escherichia coli