Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.6 extracted from

  • Lu, G.; Bluemling, G.R.; Mao, S.; Hager, M.; Gurale, B.P.; Collop, P.; Kuiper, D.; Sana, K.; Painter, G.R.; De La Rosa, A.; Kolykhalov, A.A.
    Simple in vitro assay to evaluate the incorporation efficiency of ribonucleotide analog 5-triphosphates into RNA by human mitochondrial DNA-dependent RNA polymerase (2018), Antimicrob. Agents Chemother., 62, e01830-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2'-C-ethynyl-7-deaza-ATP causes immediate chain termination Homo sapiens
2'-C-Me-ATP causes immediate chain termination Homo sapiens
3'-dATP causes immediate chain termination Homo sapiens
ara-ATP causes partial chain termination Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RNAn Homo sapiens
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00411
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNAn
-
 Homo sapiens diphosphate + RNAn+1
-
 ?
ATP + RNAn the primer can be extended only in the presence of ATP. The length of the double-stranded region of RNA/DNA duplexes is important for the primer extension. The length of the double-stranded region of RNA/DNA duplexes is important for the primer extension performed by the enzyme. Under these in vitro conditions POLRMT has a preference for P/T duplexes with short hybridization regions Homo sapiens diphosphate + RNAn+1
-
 ?
additional information incorporation of 2'-dATP or 2'-F-ATP does not lead to immediate chain termination, but the enzyme can not add the second 2'-dATP or 2'-F-ATP to the synthesized RNA Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
DNA-dependent RNA polymerase
-
 Homo sapiens
POLRMT
-
 Homo sapiens