Cloned (Comment) | Organism |
---|---|
RPB2 gene encoding the Rpb2 subunit of yeast RNAP II, expression of mutant enzyme and subunits in Escherichia coli strain XL-1 Blue | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D505A | mutation in subunit Rpb2, the mutant shows a weak defect in the escape from a transcriptional stall at A20 | Saccharomyces cerevisiae |
E1028Q | mutation in subunit Rpb2, the mutant shows transcription elongation defects | Saccharomyces cerevisiae |
E529A | mutation in subunit Rpb2, the mutant is faster in elongation compared to wild type RNAP II | Saccharomyces cerevisiae |
E529D | mutation in subunit Rpb2, the mutant is faster in elongation compared to wild type RNAP II | Saccharomyces cerevisiae |
G985A/G987A | the double substitution in subunit Rpb2 is expected to subtly affect the conformation and/or dynamics of K987, an essential residue | Saccharomyces cerevisiae |
K979Q | lethal mutation in subunit Rpb2 | Saccharomyces cerevisiae |
K979R | lethal mutation in subunit Rpb2 | Saccharomyces cerevisiae |
K987Q | lethal mutation in subunit Rpb2 | Saccharomyces cerevisiae |
K987R | lethal mutation in subunit Rpb2 | Saccharomyces cerevisiae |
additional information | mutations are made in highly conserved residues Rpb2 K979, K987, and R1020 within the active site region of RNAP II, at positions that potentially might be essential for catalytic function. Mutations are constructed in an extended fork region of subunit Rpb2, residues R504-E529, construction of diverse mutant strains, e.g. strain yBC-9 expressing subunit Rpb2 under the control of the Gal promoter and is deleted for the chromosomal copy of the DST1 gene, encoding transcription factor IIS, TFIIS. From the strain yBC-9, the strain yBC-32 was constructed by transformation with pFL39-RPB2-TAP, TRP1 | Saccharomyces cerevisiae |
Q513A | mutation in subunit Rpb2, the mutant shows a weak defect in the escape from a transcriptional stall at A20 | Saccharomyces cerevisiae |
R1020K | lethal mutation in subunit Rpb2 | Saccharomyces cerevisiae |
R1020Q | lethal mutation in subunit Rpb2 | Saccharomyces cerevisiae |
R512A | mutation in subunit Rpb2, the mutant shows transcription elongation defects | Saccharomyces cerevisiae |
R512C | mutation in subunit Rpb2, the mutant shows transcription elongation defects | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae yBC-10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant RNAP II proteins | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
dodecamer | the Rpb2 subunit of RNAP II combines with the Rpb1 subunit to form the RNAP II active site. RNAP II is isolated as a 12 subunit enzyme, Rpb1-Rpb12 | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
RNA polymerase II | - |
Saccharomyces cerevisiae |
RNAP II | - |
Saccharomyces cerevisiae |