Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sigma factor | required for activity | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rifampicin | - |
Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RNAP rom Mycobacterium smegmatis from exponential phase | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the RNAP purified from exponential phase shows low promoter specificity in promoter-polymerase interaction studies due to the presence of a large number of sigma factors during exponential phase and under-representation of sigma A required for house-keeping transcription | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | the RNAP purified from exponential phase shows low promoter specificity in promoter-polymerase interaction studies due to the presence of a large number of sigma factors during exponential phase and under-representation of sigma A required for house-keeping transcription | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
nucleoside triphosphate + RNAn | in vitro transcription with calf thymus DNA as template | Mycolicibacterium smegmatis | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | in vitro transcription with calf thymus DNA as template | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA polymerase | - |
Mycolicibacterium smegmatis |
RNAP | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycolicibacterium smegmatis |