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Literature summary for 2.7.7.6 extracted from
- Isolation and physical properties of the DNA-directed RNA polymerase from Thermus thermophilus HB8 (1990), Eur. J. Biochem., 191, 467-472.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermidine | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus |  |
| thermine | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| MnCl2 | in presence of 10 mM MgCl2 | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus | |
| Mg2+ | optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
| 92000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
| 140000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
| 170000 | - |
betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
HB8 | - |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
HB8 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.03 | - |
- |
Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | calf thymus DNA as template | Thermus thermophilus | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Thermus thermophilus | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | calf thymus DNA as template | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | betax,betax,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermus thermophilus |
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