Cloned (Comment) | Organism |
---|---|
gene glnD, overexpression from plasmid in an Escherichia coli strain | Escherichia coli |
General Stability | Organism |
---|---|
freezing and thawing of the purified enzyme results in 35-50% loss of activity | Escherichia coli |
purification drastically reduces enzyme stability | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | inhibits uridylyl removing activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
monomeric enzyme form, HPLC gel filtration | Escherichia coli |
186000 | - |
dimeric enzyme form, HPLC gel filtration | Escherichia coli |
550000 | - |
hexameric form, HPLC gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-PII] | Escherichia coli | the enzyme is regulated by Gln and controls the activity of PII signal transduction protein | diphosphate + uridylyl-[protein-PII] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene glnD | - |
Purification (Comment) | Organism |
---|---|
1720fold to homogeneity from recombinant overexpressing strain | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, several months without loss of activity | Escherichia coli |
4°C, purified enzyme, in presence of ATP and 0.2 M KCl, 10 h, loss of 15-20% activity compared to storage conditions at -5°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-PII] | - |
Escherichia coli | diphosphate + uridylyl-[protein-PII] | - |
r | |
UTP + [protein-PII] | the enzyme is regulated by Gln and controls the activity of PII signal transduction protein | Escherichia coli | diphosphate + uridylyl-[protein-PII] | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS | Escherichia coli |
hexamer | 6 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS | Escherichia coli |
monomer | 1 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS | Escherichia coli |