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Literature summary for 2.7.7.53 extracted from

  • McLennan, A.G.; Mayers, E.; Adams, D.G.
    Anabaena flos-aquae and other cyanobacteria possess diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) phosphorylase activity (1996), Biochem. J., 320, 795-800.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.53

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 P1,P4-bis(5'-adenosyl) tetraphosphate pH 9.5 Dolichospermum flos-aquae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Dolichospermum flos-aquae
Co2+ activates Dolichospermum flos-aquae
Mg2+ required, most effective divalent cation Dolichospermum flos-aquae
Mn2+ activates Dolichospermum flos-aquae
Zn2+ slight activation Dolichospermum flos-aquae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Dolichospermum flos-aquae
32000
-
 gel filtration Trichormus variabilis
39000
-
 gel filtration Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Dolichospermum flos-aquae
-
-
-
Synechococcus sp.
-
-
-
Trichormus variabilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 206fold Dolichospermum flos-aquae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.037
-
 partially purified enzyme Dolichospermum flos-aquae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information P1,P6-bis(5'-adenosyl) hexaphosphate is a very poor substrate Dolichospermum flos-aquae ?
-
 ?
additional information no activity with sulfate Dolichospermum flos-aquae ?
-
 ?
P1,P2-bis(5'-adenosyl) diphosphate + phosphate no activity Dolichospermum flos-aquae ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
-
 Dolichospermum flos-aquae ADP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate 182% activity compared to phosphate Dolichospermum flos-aquae ?
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate poor substrate Dolichospermum flos-aquae ?
-
 r
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate poor substrate Dolichospermum flos-aquae ?
-
 r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Dolichospermum flos-aquae ADP + ATP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Synechococcus sp. ADP + ATP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Trichormus variabilis ADP + ATP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate no activity Dolichospermum flos-aquae ?
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate 225% activity compared to phosphate Dolichospermum flos-aquae ?
-
 r
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
-
 Dolichospermum flos-aquae ADP + p4A
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Dolichospermum flos-aquae