Cloned (Comment) | Organism |
---|---|
- |
Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
C83F | 230% of wild-type activity for transfer to terminal U residue | Trypanosoma brucei |
D97A | complete inactivation of uridylyl transfer | Trypanosoma brucei |
K149A | mutant retains partial activity | Trypanosoma brucei |
R141A | almost complete inactivation of uridylyl transfer | Trypanosoma brucei |
R144A | almost complete inactivation of uridylyl transfer | Trypanosoma brucei |
R435A | almost complete inactivation of uridylyl transfer | Trypanosoma brucei |
V271R | 10-20% of wild-type activity | Trypanosoma brucei |
V271R/C83F | 240% of wild-type activity for transfer to terminal U residue | Trypanosoma brucei |
Y319A | complete inactivation of uridylyl transfer | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
UTP | mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0006 | - |
UTP | mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0025 | - |
UTP | wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0026 | - |
UTP | mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0028 | - |
UTP | mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.003 | - |
UTP | mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0081 | - |
UTP | mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0126 | - |
UTP | wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0209 | - |
UTP | mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + RNAn | invariant arginine residues 144 and 435 positioned in the vicinity of the UTP-binding site are critical for isoform RET2 activity on single-stranded and double-stranded RNAs, as well as function in vivo. Recognition of a double-stranded RNA, which resembles a guide RNA/mRNA duplex, is further facilitated by multipoint contacts across the RET2-specific middle domain | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn containing a terminal G residue | substrate dsRNA | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn containing a terminal U residue | substrate dsRNA | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RET2 | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
UTP | mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.00003 | - |
UTP | mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.00005 | - |
UTP | mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.00012 | - |
UTP | mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0003 | - |
UTP | wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0012 | - |
UTP | mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0015 | - |
UTP | mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0018 | - |
UTP | mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.003 | - |
UTP | wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
UTP | mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0058 | - |
UTP | mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0097 | - |
UTP | mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0567 | - |
UTP | mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.0867 | - |
UTP | mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.117 | - |
UTP | wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.21 | - |
UTP | mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.25 | - |
UTP | wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei | |
0.6 | - |
UTP | mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication | Trypanosoma brucei |