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Literature summary for 2.7.7.52 extracted from

  • Ringpis, G.E.; Aphasizheva, I.; Wang, X.; Huang, L.; Lathrop, R.H.; Hatfield, G.W.; Aphasizhev, R.
    Mechanism of U insertion RNA editing in trypanosome mitochondria: the bimodal TUTase activity of the core complex (2010), J. Mol. Biol., 399, 680-695.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.52

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 RNAn RNA editing core complex RECC, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0025
-
 RNAn recombinant enzyme, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0126
-
 RNAn containing a terminal U residue recombinant enzyme, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0208
-
 RNAn containing a terminal U residue RNA editing core complex RECC, pH 8.0, temperature not specified in the publication Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Trypanosoma brucei 5739
-

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant RET2 catalyzes a faithful editing on gapped precleaved double-stranded RNA substrates, and this reaction requires an internal monophosphate group at the 5' end of the mRNA 3' cleavage fragment. RET2 processivity is limited to insertion of three U residues. Incorporation into the RNA editing core complex RECC allows filling of longer gaps similar to those observed in vivo. Monomeric and RECC-embedded enzymes display a similar bimodal activity, the distributive insertion of a single uracil is followed by a processive extension limited by the number of guiding nucleotides. The distributive +1 insertion creates a substrate for the processive gap-filling reaction. Upon base-pairing of the +1 extended 5' cleavage fragment with a guiding nucleotide, this substrate is recognized by RET2 in a different mode compared to the product of the initial nucleolytic cleavage. Therefore, RET2 distinguishes base pairs in gapped RNA substrates Trypanosoma brucei ?
-
 ?
UTP + RNAn
-
 Trypanosoma brucei diphosphate + RNAn+1
-
 ?
UTP + RNAn containing a terminal U residue
-
 Trypanosoma brucei diphosphate + RNAn+1
-
 ?

Subunits

Subunits Comment Organism
More isoform RET2 is an integral component of the RNA editing core complex RECC Trypanosoma brucei

Synonyms

Synonyms Comment Organism
RET2
-
 Trypanosoma brucei
RNA editing TUTase
-
 Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 RNAn recombinant enzyme, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0007
-
 RNAn RNA editing core complex RECC, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0015
-
 RNAn containing a terminal U residue RNA editing core complex RECC, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0033
-
 RNAn containing a terminal U residue recombinant enzyme, pH 8.0, temperature not specified in the publication Trypanosoma brucei

General Information

General Information Comment Organism
physiological function isoform RET2 is an integral component of the RNA editing core complex RECC. Interaction of RET2 with RECC is accomplished via a protein-protein contact between its middle domain and structural subunit MP81. The recombinant RET2 catalyzes a faithful editing on gapped precleaved double-stranded RNA substrates, and this reaction requires an internal monophosphate group at the 5' end of the mRNA 3' cleavage fragment. RET2 processivity is limited to insertion of three U residues. Incorporation into the RECC voids the internal phosphate requirement and allows filling of longer gaps similar to those observed in vivo. Monomeric and RECC-embedded enzymes display a similar bimodal activity, the distributive insertion of a single uracil is followed by a processive extension limited by the number of guiding nucleotides Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.067
-
 RNAn containing a terminal U residue RNA editing core complex RECC, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.133
-
 RNAn recombinant enzyme, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.167
-
 RNAn RNA editing core complex RECC, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.267
-
 RNAn containing a terminal U residue recombinant enzyme, pH 8.0, temperature not specified in the publication Trypanosoma brucei