Cloned (Comment) | Organism |
---|---|
expression in HeLa cells via vaccinia virus strain WR into whose thymidine kinase gene the reovirus lambda2 genome segment has been inserted, possesses neither nucleoside nor RNA 5'-triphosphatase activity nor methyltransferase activity | Mammalian orthoreovirus 3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalian orthoreovirus 3 | - |
- |
- |
Mammalian orthoreovirus 3 | - |
from infected mouse fibroblasts | - |
Mammalian orthoreovirus 3 Dearing | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from vaccinia virus grown in HeLa cells | Mammalian orthoreovirus 3 |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 50% glycerol, 0.1 mg/ml gelatin, enzyme concentration: 0.1 mg/ml, stable | Mammalian orthoreovirus 3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | reversible formation of a covalent intermediate enzyme-GMP | Mammalian orthoreovirus 3 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | reversible formation of a covalent intermediate enzyme-GMP | Mammalian orthoreovirus 3 Dearing | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
additional information | enzyme also catalyzes GTP-diphosphate exchange | Mammalian orthoreovirus 3 | ? | - |
? | |
additional information | enzyme also catalyzes GTP-diphosphate exchange | Mammalian orthoreovirus 3 Dearing | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | native enzyme forms a pentamer, recombinant enzyme from vaccinia virus forms a monomer | Mammalian orthoreovirus 3 |