Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 80000, beta, RNA 5-triphosphatase + x * 52000, alpha, mRNA guanylyltransferase activity, SDS-PAGE | Saccharomyces cerevisiae |
80000 | - |
x * 80000, beta, RNA 5-triphosphatase + x * 52000, alpha, mRNA guanylyltransferase activity, SDS-PAGE | Saccharomyces cerevisiae |
180000 | - |
glycerol density gradient sedimentation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | mRNA capping enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large scale, to homogeneity, enzyme has mRNA guanylyltransferase activity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.27 | - |
purified enzyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | - |
Saccharomyces cerevisiae | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
additional information | mRNA capping enzyme | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, beta, RNA 5'-triphosphatase + x * 52000, alpha, mRNA guanylyltransferase activity, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
capping enzyme | - |
Saccharomyces cerevisiae |
mRNA capping enzyme | has mRNA guanylyltransferase and RNA 5'-triphosphatase activity | Saccharomyces cerevisiae |