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Literature summary for 2.7.7.50 extracted from

  • Monroy, G.; Spencer, E.; Hurwitz, J.
    Purification of mRNA guanylyltransferase from vaccinia virions (1978), J. Biol. Chem., 253, 4481-4489.
    View publication on PubMed
Search for more literature for 2.7.7.50

Localization

Localization Comment Organism GeneOntology No. Textmining
viral nucleocapsid
-
 Vaccinia virus 19013
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 x * 59000, guanylyltransferase lacking 7-methyltransferase activity, SDS-PAGE Vaccinia virus
120000
-
 copurifies with S-adenosylmethionine mRNA (guanine-7)-methyltransferase, sucrose density gradient centrifugation and gel filtration Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
copurifies with S-adenosylmethionine mRNA (guanine-7)-methyltransferase Vaccinia virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 purified enzyme Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
 Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
GTP + pp(5')RNA
-
 Vaccinia virus WR G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?

Subunits

Subunits Comment Organism
? x * 59000, guanylyltransferase lacking 7-methyltransferase activity, SDS-PAGE Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Vaccinia virus