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Literature summary for 2.7.7.5 extracted from

  • Grunberg-Manago, M.; Del Campillo-Campbell, A.; Dondon, L.; Michelson, A.M.
    Yeast ADP-sulfurylase catalyzing an exchange between orthophosphate and the terminal phosphate of nucleoside diphosphates (1966), Biochim. Biophys. Acta, 123, 1-16.
    View publication on PubMed
Search for more literature for 2.7.7.5

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
 Saccharomyces fragilis

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Saccharomyces fragilis
arsenate
-
 Saccharomyces fragilis
chromate
-
 Saccharomyces fragilis
F-
-
 Saccharomyces fragilis
NaCl slight inhibition Saccharomyces fragilis
nitrate
-
 Saccharomyces fragilis
p-chloromercuribenzoate
-
 Saccharomyces fragilis
selenate
-
 Saccharomyces fragilis
vanadate
-
 Saccharomyces fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 GDP 30°C Saccharomyces fragilis
0.0005
-
 ADP 30°C Saccharomyces fragilis
0.0015
-
 phosphate 30°C Saccharomyces fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slight inhibition Saccharomyces fragilis
Ca2+ strong inhibition Saccharomyces fragilis
Cu2+ strong inhibition Saccharomyces fragilis
Fe2+ strong inhibition Saccharomyces fragilis
Mn2+ strong inhibition Saccharomyces fragilis
Zn2+ strong inhibition Saccharomyces fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphate + adenosine 5'-phosphosulfate Saccharomyces fragilis
-
 ADP + SO42-
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces fragilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
 purified enzyme, 30°C Saccharomyces fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate
-
 Saccharomyces fragilis phosphate + ADP
-
 ?
CDP + phosphate
-
 Saccharomyces fragilis phosphate + CDP
-
 ?
GDP + phosphate
-
 Saccharomyces fragilis phosphate + GDP
-
 ?
IDP + phosphate
-
 Saccharomyces fragilis phosphate + IDP
-
 ?
additional information several nucleotide derivatives tested for activity Saccharomyces fragilis ?
-
 ?
phosphate + adenosine 5'-phosphosulfate
-
 Saccharomyces fragilis ADP + SO42-
-
 ?
UDP + phosphate
-
 Saccharomyces fragilis phosphate + UDP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Saccharomyces fragilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 complete loss of activity within 30 min Saccharomyces fragilis
40
-
 71% loss of activity within 5 min Saccharomyces fragilis
50
-
 84% loss of activity within 1 min Saccharomyces fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Saccharomyces fragilis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 with ADP, CDP and GDP Saccharomyces fragilis
5.5 7.5 rapid loss of activity above pH 7.5 with UDP Saccharomyces fragilis