Protein Variants | Comment | Organism |
---|---|---|
A502V | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
A644V | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
D200N | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
D200N/L603W/T330P/L139P/E607K | site-directed mutagenesis, highly processive and thermostable multiply-mutated M-MuLV RT variant with 65fold improvement in comparison to the wild-type enzyme, the maximum temperature of the full-length cDNA synthesis is raised to 62°C, compared to 45°C for the wild-type enzyme | Moloney murine leukemia virus |
E607K | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
F625S | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
H126R | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
K658R | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
L139P | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
L333Q | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
L603W | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
L671P | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
M428L | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
additional information | generation of enzyme mutants with increased thermostability using compartmentalized ribosome display evolution in vitro technique, overview. Identification of a large set of mutations that enable cDNA synthesis at elevated temperatures. Altered substrate-binding affinity and progressivity of mutant enzymes, overview | Moloney murine leukemia virus |
N649S | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
P130S | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
P65S | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
T287A | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
T330P | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
W388R | site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
Y344H | site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme | Moloney murine leukemia virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Moloney murine leukemia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moloney murine leukemia virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNA-DNA hybrid substrate preparation by annealing the RNA oligonucleotide LA-237 with a 1.2fold excess of the complementary DNA oligonucleotide JV-08 | Moloney murine leukemia virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M-MuLV reverse transcriptase | - |
Moloney murine leukemia virus |
Moloney Murine leukemia virus reverse transcriptase | - |
Moloney murine leukemia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Moloney murine leukemia virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
thermal inactivation half-life of point mutants at 50°C, overview | Moloney murine leukemia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Moloney murine leukemia virus |