Protein Variants | Comment | Organism |
---|---|---|
K65R | site-directed mutagenesis | Human immunodeficiency virus 1 |
L74V | site-directed mutagenesis | Human immunodeficiency virus 1 |
M184V | site-directed mutagenesis | Human immunodeficiency virus 1 |
Q151M | site-directed mutagenesis | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-3'-azido-2',3'-dideoxy-N,N-dimethyladenosine 5'-triphosphate | - |
Human immunodeficiency virus 1 | |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | - |
Human immunodeficiency virus 1 | |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | - |
Human immunodeficiency virus 1 | |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | - |
Human immunodeficiency virus 1 | |
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | - |
Human immunodeficiency virus 1 | |
3'-azido-2',3'-dideoxyadenosine triphosphate | - |
Human immunodeficiency virus 1 | |
3'-azido-2',3'-dideoxyguanosine triphosphate | - |
Human immunodeficiency virus 1 | |
additional information | EC50 values, molecular modeling, overview | Human immunodeficiency virus 1 | |
zidovudine triphosphate | - |
Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state incorporation of 6-modified 3'-azido-ddGTP nucleotides by HIV-1 RT, steady-state kinetics, overview | Human immunodeficiency virus 1 | |
0.00017 | - |
dGTP | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00029 | - |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00032 | - |
3'-azido-2',3'-dideoxyadenosine triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00033 | - |
dATP | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00092 | - |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0018 | - |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0048 | - |
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.012 | - |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0163 | - |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.034 | - |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Human immunodeficiency virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | Human immunodeficiency virus 1 | RNA-dependent DNA synthesis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Human immunodeficiency virus 1 | RNA-dependent DNA synthesis | diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | Human immunodeficiency virus 1 | RNA-dependent DNA synthesis | diphosphate + DNAn+1 | - |
? | |
additional information | Human immunodeficiency virus 1 | the enzyme performs DNA-dependent DNA synthesis and RNA-dependent DNA synthesis, see also EC 2.7.7.7 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn | incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue | Human immunodeficiency virus 1 | diphosphate + ? | - |
? | |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn | incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue | Human immunodeficiency virus 1 | diphosphate + ? | - |
? | |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn | incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue | Human immunodeficiency virus 1 | diphosphate + ? | - |
? | |
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn | incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue | Human immunodeficiency virus 1 | diphosphate + ? | - |
? | |
3'-azido-2',3'-dideoxyadenosine triphosphate + DNAn | incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue | Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
dATP + DNAn | RNA-dependent DNA synthesis | Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | RNA-dependent DNA synthesis | Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | RNA-dependent DNA synthesis | Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? | |
additional information | the enzyme performs DNA-dependent DNA synthesis and RNA-dependent DNA synthesis, see also EC 2.7.7.7 | Human immunodeficiency virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HIV-1 RT | - |
Human immunodeficiency virus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human immunodeficiency virus 1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.22 | - |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.37 | - |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
2.1 | - |
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
2.7 | - |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
2.8 | - |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
14 | - |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
14 | - |
3'-azido-2',3'-dideoxyadenosine triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
17 | - |
dATP | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
17.3 | - |
dGTP | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Human immunodeficiency virus 1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00006 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyguanosine triphosphate | |
0.00012 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | zidovudine triphosphate | |
0.00013 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | zidovudine triphosphate | |
0.00015 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyguanosine triphosphate | |
0.00016 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyadenosine triphosphate | |
0.00018 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | zidovudine triphosphate | |
0.00019 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyadenosine triphosphate | |
0.00025 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyguanosine triphosphate | |
0.0003 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyguanosine triphosphate | |
0.00034 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.00036 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyguanosine triphosphate | |
0.00042 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyadenosine triphosphate | |
0.00066 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | zidovudine triphosphate | |
0.0007 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyadenosine triphosphate | |
0.0008 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0013 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0014 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxyadenosine triphosphate | |
0.0025 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0025 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0027 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | zidovudine triphosphate | |
0.0032 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0041 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0042 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0048 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0088 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0091 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0103 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0118 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0218 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0234 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 2-amino-3'-azido-2',3'-dideoxy-N,N-dimethyladenosine 5'-triphosphate | |
0.0244 | - |
pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0308 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.0329 | - |
pH 7.5, 37°C, mutant M184V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.05 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 2-amino-3'-azido-2',3'-dideoxy-N,N-dimethyladenosine 5'-triphosphate | |
0.05 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 2-amino-3'-azido-2',3'-dideoxy-N,N-dimethyladenosine 5'-triphosphate | |
0.05 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 2-amino-3'-azido-2',3'-dideoxy-N,N-dimethyladenosine 5'-triphosphate | |
0.078 | - |
pH 7.5, 37°C, mutant Q151M | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.08 | - |
pH 7.5, 37°C, mutant K65R | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | |
0.1 | - |
pH 7.5, 37°C, mutant L74V | Human immunodeficiency virus 1 | 3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.000013 | - |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00023 | - |
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.000402 | - |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00044 | - |
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00102 | - |
dGTP | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0015 | - |
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.044 | - |
3'-azido-2',3'-dideoxyadenosine triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.048 | - |
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.052 | - |
dATP | pH 7.5, 37°C, wild-type enzyme | Human immunodeficiency virus 1 |