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Literature summary for 2.7.7.49 extracted from

  • Pandeya, D.; Yang, Y.; Hong, S.
    Active recombinant reverse transcriptase domain of human hepatitis B virus polymerase (2011), Biomed. Res., 22, 381-386.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of active polyhistidine-tagged reverse transcriptase domain, comprising residues 304-693, in an Escherichia coli/rabbit reticulocyte lysate coupled transcriptase-translation system Hepatitis B virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Hepatitis B virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Hepatitis B virus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Hepatitis B virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant polyhistidine-tagged reverse transcriptase domain from Escherichia coli by nickel affinity chromatography Hepatitis B virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Hepatitis B virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn poly(dA)/oligo(dT)12-18 and poly(rA)/oligo(dT)12-18 as template-primers Hepatitis B virus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
HBV-pol
-
Hepatitis B virus
human hepatitis B virus polymerase
-
Hepatitis B virus
polymerase/reverse transcriptase
-
Hepatitis B virus
reverse transcriptase
-
Hepatitis B virus
reverse transcriptase/RNA dependent DNA polymerase
-
Hepatitis B virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Hepatitis B virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Hepatitis B virus

General Information

General Information Comment Organism
additional information the purified recombinant reverse transcriptase domain is a stable protein and shows a low selective polymerase activity, substrate binding by computational molecular modeling, overview Hepatitis B virus
physiological function hepatitis B virus polymerase plays a critical role during HBV life cycle, and polymerase/reverse transcriptase activities are critical for HBV-pol during viral replication Hepatitis B virus