Literature summary for 2.7.7.49 extracted from

Lu, M.; Ngo, W.; Mei, Y.; Munshi, V.; Burlein, C.; Loughran, M.; Williams, P.; Hazuda, D.; Miller, M.; Grobler, J.; Diamond, T.; Lai, M.
Purification of untagged HIV-1 reverse transcriptase by affinity chromatography (2010), Protein Expr. Purif., 71, 231-239.

Application

Application	Comment	Organism
drug development	the enzyme is a primary target in the development of antiviral agents against HIV-1	Human immunodeficiency virus 1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of untagged or C-terminally intein- and biotin-tagged enzyme in Escherichia coli strain BL21 (DE3)	Human immunodeficiency virus 1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Human immunodeficiency virus 1

Organism

Organism	UniProt	Comment	Textmining
Human immunodeficiency virus 1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification method development, one-step purification 157fold using an affinity column prepared by conjugating an RNase H specific inhibitor with NHS-activated resin, washing with Mn2+, and elution with EDTA, or purification using a C-terminal intein and a biotin tag for avidin affinty chromatography, followed by reductive cleavage of the Mxe intein, overview. Purification of recombinant enzyme from Escherichia coli strain BL21 (DE3). RNase inhibitors have a greater affinity for the RNase H active site in the presence of Mn2+ than in the presence of Mg2+	Human immunodeficiency virus 1

Purification (Comment)

Organism

purification method development, one-step purification 157fold using an affinity column prepared by conjugating an RNase H specific inhibitor with NHS-activated resin, washing with Mn2+, and elution with EDTA, or purification using a C-terminal intein and a biotin tag for avidin affinty chromatography, followed by reductive cleavage of the Mxe intein, overview. Purification of recombinant enzyme from Escherichia coli strain BL21 (DE3). RNase inhibitors have a greater affinity for the RNase H active site in the presence of Mn2+ than in the presence of Mg2+

Human immunodeficiency virus 1

Synonyms

Synonyms	Comment	Organism
HIV-1 R	-	Human immunodeficiency virus 1
HIV-1 reverse transcriptase	-	Human immunodeficiency virus 1
human immunodeficiency virus type 1 reverse transcriptase	-	Human immunodeficiency virus 1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Human immunodeficiency virus 1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Human immunodeficiency virus 1

General Information

General Information	Comment	Organism
physiological function	HIV-1 reverse transcriptase plays an essential role in the life cycle of the virus	Human immunodeficiency virus 1