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Literature summary for 2.7.7.49 extracted from

  • Bahrami, F.; Jestin, J.L.
    Streptococcus agalactiae DNA polymerase I is an efficient reverse transcriptase (2008), Biochimie, 90, 1796-1799.
    View publication on PubMed
Search for more literature for 2.7.7.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Bli5 Streptococcus agalactiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 deoxy-5'-CTP at 37°C Streptococcus agalactiae
0.0021
-
 2'-deoxyadenosine 5'-triphosphate at 37°C Streptococcus agalactiae
0.0046
-
 2'-deoxythymidine 5'-triphosphate at 37°C Streptococcus agalactiae
0.028
-
 2'-deoxyguanosine 5'-triphosphate at 37°C Streptococcus agalactiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
 gel filtration Streptococcus agalactiae

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae
-
-
-
Streptococcus agalactiae NEM316
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Streptococcus agalactiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae diphosphate + DNAn+1
-
 ?
2'-deoxyadenosine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae NEM316 diphosphate + DNAn+1
-
 ?
2'-deoxycytidine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae diphosphate + DNAn+1
-
 ?
2'-deoxycytidine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae NEM316 diphosphate + DNAn+1
-
 ?
2'-deoxyguanosine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae diphosphate + DNAn+1
-
 ?
2'-deoxyguanosine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae NEM316 diphosphate + DNAn+1
-
 ?
2'-deoxythymidine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae diphosphate + DNAn+1
-
 ?
2'-deoxythymidine 5'-triphosphate + DNAn
-
 Streptococcus agalactiae NEM316 diphosphate + DNAn+1
-
 ?
additional information no saturation is observed for extension on DNA templates Streptococcus agalactiae ?
-
 ?
additional information no saturation is observed for extension on DNA templates Streptococcus agalactiae NEM316 ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase I
-
 Streptococcus agalactiae
RNA-dependent DNA polymerase
-
 Streptococcus agalactiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0117
-
 deoxy-5'-CTP at 37°C Streptococcus agalactiae
0.0265
-
 2'-deoxyadenosine 5'-triphosphate at 37°C Streptococcus agalactiae
0.034
-
 2'-deoxythymidine 5'-triphosphate at 37°C Streptococcus agalactiae
1.33
-
 2'-deoxyguanosine 5'-triphosphate at 37°C Streptococcus agalactiae