Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.49 extracted from

  • Vellore, J.; Moretz, S.E.; Lampson, B.C.
    A group II intron-type open reading frame from the thermophile Bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase (2004), Appl. Environ. Microbiol., 70, 7140-7147.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
encoded by group II intron-type open reading frame
-

Purification (Commentary)

Purification (Comment) Organism
partial Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Geobacillus stearothermophilus diphosphate + DNAn+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
activity increases from 37°C, reaching its highest activity at 65°C Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 65 activity increases from 37°C, reaching its highest activity at 65°C, significant decrease in activity at 75°C Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
heat stable enzyme Geobacillus stearothermophilus