Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.49 extracted from

  • Piskareva, O.; Denmukhametova, S.; Schmatchenko, V.
    Functional reverse transcriptase encoded by the human LINE-1 from baculovirus-infected insect cells (2003), Protein Expr. Purif., 28, 125-130.
    View publication on PubMed
Search for more literature for 2.7.7.49

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 10 mM dithiothreitol Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
human LINE-1 ORF2, which encodes reverse transcriptase, is inserted into a baculovirus shuttle vector and expressed in SF21 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ above 1 mM MnCl2 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 50 mM KCl Homo sapiens
Mg2+ divalent metal ion required, enzyme has a preference for Mg2+ over Mn2+. Activity on poly(rA)*oligo(dT)12 is almost 3fold higher with Mg2+ than with Mn2+ Homo sapiens
Mn2+ divalent metal ion required, enzyme has a preference for Mg2+ over Mn2+. Activity on poly(rA)*oligo(dT)12 is almost 3fold higher with Mg2+ than with Mn2+. Maximal dTTP incorporation into poly(rA)*oligo(dT)12 occurs between 0.3 and 0.6 mM MnCl2 Homo sapiens
NaCl maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 at 100 mM NaCl Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human LINE-1 ORF2, which encodes reverse transcriptase, is inserted into a baculovirus shuttle vector and expressed in SF21 cells
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, 50% glycerol, stable for more than 12 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn DNA synthesis of the recombinant enzyme is higher on poly(rA)*oligo(dT)12 than on poly(rC)*oligo(dG). The activity on poly[d(A-T)] is noticeably lower than that on poly(rA)*oligo(dT)12 Homo sapiens diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 47 23°C: about 30% of maximal activity, 47°C: about 15% of maximal activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 Homo sapiens