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Literature summary for 2.7.7.49 extracted from

  • Luke, W.; Hoefer, K.; Moosmayer, D.; Nickel, P.; Hunsmann, G.; Jentsch, K.D.
    Partial purification and characterization of the reverse transcriptase of the simian immunodeficiency virus TYO-7 isolated from an African green monkey (1990), Biochemistry, 29, 1764-1769.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Dextran sulfate IC50: 0.0044 mg/ml Simian immunodeficiency virus
heparin IC50: 0.0740 mg/ml Simian immunodeficiency virus
NF 345 IC50: 7 mg/ml Simian immunodeficiency virus
NF 346 IC50: 5 mg/ml Simian immunodeficiency virus
suramin IC50: 4 mg/ml Simian immunodeficiency virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Simian immunodeficiency virus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimal activity at 50 mM KCl with poly(rA)*oligo(dT) and 10-100 mM KCl for poly(rC)*oligo(dG) Simian immunodeficiency virus
Mg2+ highest activity is obtained at 5 mM Mg2+ with poly(rA)*oligo(dI) and at about 15 mM Mg2+ with poly(rC)*oligo(dG) and at 15 mM Mg2+ with poly(rC)*oligo(dG) Simian immunodeficiency virus
Mn2+ almost no activity is detected in the presence of Mn2+ Simian immunodeficiency virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
1 * 50000 + 1 * 64000, both subunits exhibit enzymatic activity, the 64000 Da subunit shows the predominant activity, SDS-PAGE Simian immunodeficiency virus
64000
-
1 * 50000 + 1 * 64000, both subunits exhibit enzymatic activity, the 64000 Da subunit shows the predominant activity, SDS-PAGE Simian immunodeficiency virus
120000 130000 glycerol gradient centrifugation Simian immunodeficiency virus

Organism

Organism UniProt Comment Textmining
Simian immunodeficiency virus
-
TYO-7, isolated from an African monkey
-

Purification (Commentary)

Purification (Comment) Organism
partial Simian immunodeficiency virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.135
-
incorporation of dTMP with poly(RA)*oligo(dT) as template-primer Simian immunodeficiency virus

Storage Stability

Storage Stability Organism
4°C, after 2 d the enzyme loses 20% of the poly(rA)*oligo(dT) activity, no activity loss is observed with poly(rC)*oligo(dG) activity Simian immunodeficiency virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme prefers the template-primer poly(rA)*oligo(dT) over poly(rC)*oligo(dG). With poly(rCm)*oligo(dG) only marginal activity is detected, and no activity is measured with poly(dA)*oligo(dT) Simian immunodeficiency virus diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
dimer 1 * 50000 + 1 * 64000, both subunits exhibit enzymatic activity, the 64000 Da subunit shows the predominant activity, SDS-PAGE Simian immunodeficiency virus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
activity with poly(rC)*oligo(dG) is stable for up to 20 min Simian immunodeficiency virus
37
-
10 min, activity with poly(rA)*oligo(dT) is stable Simian immunodeficiency virus
45
-
25 min, poly(rA)*poligo(dT)-dependent activity is completely destroyed, 20% of the poly(rC)*oligo(dG)-dependent activity is preserved Simian immunodeficiency virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reaction with poly(rA)*oligo(dT) Simian immunodeficiency virus
8 9.5 reaction with poly(rC)*oligo(dG) Simian immunodeficiency virus