Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dextran sulfate | IC50: 0.0044 mg/ml | Simian immunodeficiency virus | |
heparin | IC50: 0.0740 mg/ml | Simian immunodeficiency virus | |
NF 345 | IC50: 7 mg/ml | Simian immunodeficiency virus | |
NF 346 | IC50: 5 mg/ml | Simian immunodeficiency virus | |
suramin | IC50: 4 mg/ml | Simian immunodeficiency virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Simian immunodeficiency virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal activity at 50 mM KCl with poly(rA)*oligo(dT) and 10-100 mM KCl for poly(rC)*oligo(dG) | Simian immunodeficiency virus | |
Mg2+ | highest activity is obtained at 5 mM Mg2+ with poly(rA)*oligo(dI) and at about 15 mM Mg2+ with poly(rC)*oligo(dG) and at 15 mM Mg2+ with poly(rC)*oligo(dG) | Simian immunodeficiency virus | |
Mn2+ | almost no activity is detected in the presence of Mn2+ | Simian immunodeficiency virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000 + 1 * 64000, both subunits exhibit enzymatic activity, the 64000 Da subunit shows the predominant activity, SDS-PAGE | Simian immunodeficiency virus |
64000 | - |
1 * 50000 + 1 * 64000, both subunits exhibit enzymatic activity, the 64000 Da subunit shows the predominant activity, SDS-PAGE | Simian immunodeficiency virus |
120000 | 130000 | glycerol gradient centrifugation | Simian immunodeficiency virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Simian immunodeficiency virus | - |
TYO-7, isolated from an African monkey | - |
Purification (Comment) | Organism |
---|---|
partial | Simian immunodeficiency virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.135 | - |
incorporation of dTMP with poly(RA)*oligo(dT) as template-primer | Simian immunodeficiency virus |
Storage Stability | Organism |
---|---|
4°C, after 2 d the enzyme loses 20% of the poly(rA)*oligo(dT) activity, no activity loss is observed with poly(rC)*oligo(dG) activity | Simian immunodeficiency virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the enzyme prefers the template-primer poly(rA)*oligo(dT) over poly(rC)*oligo(dG). With poly(rCm)*oligo(dG) only marginal activity is detected, and no activity is measured with poly(dA)*oligo(dT) | Simian immunodeficiency virus | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 50000 + 1 * 64000, both subunits exhibit enzymatic activity, the 64000 Da subunit shows the predominant activity, SDS-PAGE | Simian immunodeficiency virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity with poly(rC)*oligo(dG) is stable for up to 20 min | Simian immunodeficiency virus |
37 | - |
10 min, activity with poly(rA)*oligo(dT) is stable | Simian immunodeficiency virus |
45 | - |
25 min, poly(rA)*poligo(dT)-dependent activity is completely destroyed, 20% of the poly(rC)*oligo(dG)-dependent activity is preserved | Simian immunodeficiency virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reaction with poly(rA)*oligo(dT) | Simian immunodeficiency virus |
8 | 9.5 | reaction with poly(rC)*oligo(dG) | Simian immunodeficiency virus |