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Literature summary for 2.7.7.49 extracted from

  • Taube, R.; Loya, S.; Avidan, O.; Perach, M.; Hizi, A.
    Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization (1998), Biochem. J., 329, 579-587.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.49

Cloned(Commentary)

Cloned (Comment) Organism
construction of a plasmid that induces in bacteria the synthesis of an enzymatically active reverse transcriptase, expression of a protein with a six-histidine tag in Escherichia coli mouse mammary tumor virus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 mouse mammary tumor virus
2',3'-dideoxyguanosine triphosphate
-
 mouse mammary tumor virus
2',3'-dideoxythymidine 5'-triphosphate
-
 mouse mammary tumor virus
3'-Azido-2',3'-dideoxythymidine 5'-triphosphate
-
 mouse mammary tumor virus
KCl IC50: 40-50 mM mouse mammary tumor virus
NaCl IC50: 40-50 mM mouse mammary tumor virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for poly(rA)n*oligo(dT)12-18 is 0.004 mg/ml, Km-value for poly(rC)n*oligo(dG)12-18 is 0.0036 mg/ml, measured at pH 7.5 and 37°C mouse mammary tumor virus
0.0034
-
 dGTP pH 7.5, 37°C mouse mammary tumor virus
0.0045
-
 dTTP pH 7.5, 37°C mouse mammary tumor virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ marked preference for Mg2+ over Mn2+ mouse mammary tumor virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 glycerol gradient ultracentrifugation mouse mammary tumor virus

Organism

Organism UniProt Comment Textmining
mouse mammary tumor virus
-
 BR6 milk-transmitted strain
-
mouse mammary tumor virus BR6
-
 BR6 milk-transmitted strain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with a six-histidine tag mouse mammary tumor virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 mouse mammary tumor virus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 mouse mammary tumor virus BR6 diphosphate + DNAn+1
-
 ?
dGTP + DNAn
-
 mouse mammary tumor virus diphosphate + DNAn+1
-
 ?
dGTP + DNAn
-
 mouse mammary tumor virus BR6 diphosphate + DNAn+1
-
 ?
dTTP + poly(rA)/(dT)18
-
 mouse mammary tumor virus ?
-
 ?
dTTP + poly(rA)/(dT)18
-
 mouse mammary tumor virus BR6 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000 mouse mammary tumor virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 mouse mammary tumor virus

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 90% of maximal activity, pH 10.0: about 50% of maximal activity mouse mammary tumor virus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0085
-
 3'-Azido-2',3'-dideoxythymidine 5'-triphosphate pH 7.5, 37°C mouse mammary tumor virus
0.034
-
 2',3'-dideoxythymidine 5'-triphosphate pH 7.5, 37°C mouse mammary tumor virus
0.054
-
 2',3'-dideoxyguanosine 5'-triphosphate pH 7.5, 37°C mouse mammary tumor virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
40 50 IC50: 40-50 mM mouse mammary tumor virus NaCl
40 50 IC50: 40-50 mM mouse mammary tumor virus KCl