Any feedback?
Literature summary for 2.7.7.49 extracted from
- The reverse transcriptase (1977), Biochim. Biophys. Acta, 473, 1-38.
General Stability
| General Stability | Organism |
|---|---|
| the enzyme can be recovered after pressure dialysis | Avian myeloblastosis virus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| viral nucleocapsid | - |
murine leukemia virus | 19013 | - |
| viral nucleocapsid | - |
Moloney murine leukemia virus | 19013 | - |
| viral nucleocapsid | - |
Reticuloendotheliosis virus | 19013 | - |
| viral nucleocapsid | - |
Avian sarcoma virus | 19013 | - |
| viral nucleocapsid | - |
Spleen necrosis virus | 19013 | - |
| viral nucleocapsid | - |
Duck infectious anemia virus | 19013 | - |
| viral nucleocapsid | - |
Chicken syncytial virus | 19013 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration is 2 mM with poly(A)-oligo(dT) as template-primer and 10 mM with poly(C)-oligo(dG) or poly(dC)-oligo(dG) as template-primer | murine leukemia virus |  |
| Mg2+ | optimal concentration is 6 mM with poly(A)-oligo(dT), poly(C)-oligo(dG) or poly(dC)-oligo(dG) as template-primer | Avian myeloblastosis virus | |
| Mn2+ | optimal concentration is 1.0 mM with poly(A)-oligo(dT) as template-primer and 0.1 mM with poly(C)-oligo(dG) or poly(dC)-oligo(dG) as template-primer | Avian myeloblastosis virus | |
| Mn2+ | optimal concentration is 1.0 mM with poly(A)-oligo(dT) as template-primer and 2.0 mM with poly(C)-oligo(dG) or poly(dC)-oligo(dG) as template-primer | murine leukemia virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
1 * 53000 + 1 * 68000, SDS-PAGE | Hamster leukemia virus |
| 65000 | - |
x * 65000 + x * 95000, SDS-PAGE | Avian sarcoma virus |
| 68000 | - |
1 * 53000 + 1 * 68000, SDS-PAGE | Hamster leukemia virus |
| 95000 | - |
x * 65000 + x * 95000, SDS-PAGE | Avian sarcoma virus |
| 120000 | - |
- |
Hamster leukemia virus |
| 170000 | - |
gel filtration | Avian myeloblastosis virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Avian myeloblastosis virus | - |
diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Hamster leukemia virus | - |
diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | murine leukemia virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Moloney murine leukemia virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Reticuloendotheliosis virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Avian sarcoma virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Spleen necrosis virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Duck infectious anemia virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Chicken syncytial virus | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Reticuloendotheliosis virus T | the enzyme appears to be required very early after infection to synthesize proviral DNA | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Hamster leukemia virus HaLV | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avian myeloblastosis virus | - |
- |
- |
| Avian sarcoma virus | - |
B77 strain grown in duck embryo fibroblasts | - |
| Chicken syncytial virus | - |
- |
- |
| Duck infectious anemia virus | - |
- |
- |
| Hamster leukemia virus | - |
HaLV | - |
| Hamster leukemia virus HaLV | - |
HaLV | - |
| Macaca mulatta | - |
- |
- |
| Moloney murine leukemia virus | - |
- |
- |
| murine leukemia virus | - |
MuLV | - |
| Reticuloendotheliosis virus | - |
- |
- |
| Reticuloendotheliosis virus | - |
REV | - |
| Reticuloendotheliosis virus T | - |
- |
- |
| Spleen necrosis virus | - |
- |
- |
| Xenopus laevis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| oocyte | - |
Xenopus laevis | - |
| placenta | - |
Macaca mulatta | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Xenopus laevis | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Macaca mulatta | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Avian myeloblastosis virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Reticuloendotheliosis virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Avian sarcoma virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Spleen necrosis virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Duck infectious anemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Chicken syncytial virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Hamster leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Reticuloendotheliosis virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Avian sarcoma virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Spleen necrosis virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Duck infectious anemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Chicken syncytial virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Reticuloendotheliosis virus T | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme appears to be required very early after infection to synthesize proviral DNA | Reticuloendotheliosis virus T | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Hamster leukemia virus HaLV | diphosphate + DNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Avian myeloblastosis virus |
| ? | x * 65000 + x * 95000, SDS-PAGE | Avian sarcoma virus |
| dimer | 1 * 53000 + 1 * 68000, SDS-PAGE | Hamster leukemia virus |
| monomer | x * 70000-84000 | Reticuloendotheliosis virus |
| More | - |
Avian myeloblastosis virus |
| More | three polypeptides of 60000 Da, 68000 Da and 82000 Da are detected by SDS-PAGE. It seems likely that the 68000 Da and the 60000 Da polypeptide are degradation products of the 82000 Da polypeptide | Moloney murine leukemia virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
