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Literature summary for 2.7.7.48 extracted from

  • Hillen, H.S.; Kokic, G.; Farnung, L.; Dienemann, C.; Tegunov, D.; Cramer, P.
    Structure of replicating SARS-CoV-2 polymerase (2020), Nature, 584, 154-156 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the SARS-CoV-2 nsp12 gene is codon-optimized for expression in insect cells. The SARS-CoV-2 nsp8 and nsp7 genes are codon-optimized for expression in Escherichia coli Severe acute respiratory syndrome coronavirus 2

Inhibitors

Inhibitors Comment Organism Structure
remdesivir
-
Severe acute respiratory syndrome coronavirus 2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Severe acute respiratory syndrome coronavirus 2
-
diphosphate + RNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome coronavirus 2 P0DTD1 replicase polyprotein 1ab; SARS-CoV-2
-

Purification (Commentary)

Purification (Comment) Organism
-
Severe acute respiratory syndrome coronavirus 2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
More cryo-electron microscopy structure of the SARS-CoV-2 RdRp in an active form that mimics the replicating enzyme. The structure comprises the viral proteins non-structural protein 12 (nsp12), nsp8 and nsp7, and more than two turns of RNA template-product duplex. The active-site cleft of nsp12 binds to the first turn of RNA and mediates RdRp activity with conserved residues. Two copies of nsp8 bind to opposite sides of the cleft and position the second turn of RNA. Long helical extensions in nsp8 protrude along exiting RNA, forming positively charged sliding poles Severe acute respiratory syndrome coronavirus 2

Synonyms

Synonyms Comment Organism
RNA-dependent RNA polymerase
-
Severe acute respiratory syndrome coronavirus 2
SARS-CoV-2 RdRp
-
Severe acute respiratory syndrome coronavirus 2