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Literature summary for 2.7.7.48 extracted from

  • Li, L.; Wang, M.; Chen, Y.; Hu, T.; Yang, Y.; Zhang, Y.; Bi, G.; Wang, W.; Liu, E.; Han, J.; Lu, T.; Su, D.
    Structure of the enterovirus D68 RNA-dependent RNA polymerase in complex with NADPH implicates an inhibitor binding site in the RNA template tunnel (2020), J. Struct. Biol., 211, 107510 .
    View publication on PubMed
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Enterovirus D68

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 14°C, crystal structure of the enzyme (RdRp) in the apo state and in complex with the inhibitor NADPH Enterovirus D68

Inhibitors

Inhibitors Comment Organism Structure
NADPH the enzyme activity decreases when titrated with NADPH in vitro Enterovirus A71
NADPH the inhibitor occupies the RNA template binding channel of Human enterovirus D68 enzyme (RdRp) with a binding pocket. Residues involved in the NADPH binding pocket of EV-D68 RdRp are highly conserved in RdRps of enteroviruses. The enzyme activity decreases when titrated with NADPH in vitro Enterovirus D68

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Enterovirus A71
-
 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn Enterovirus D68
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Enterovirus A71
-
-
-
Enterovirus D68
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterovirus A71
-
 Enterovirus D68

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
 Enterovirus A71 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn
-
 Enterovirus D68 diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
RDRP
-
 Enterovirus A71
RDRP
-
 Enterovirus D68
RNA-dependent RNA polymerase
-
 Enterovirus A71
RNA-dependent RNA polymerase
-
 Enterovirus D68

General Information

General Information Comment Organism
drug target the enzyme is considered as a promising target for specific antiviral therapeutics Enterovirus A71
drug target the enzyme is considered as a promising target for specific antiviral therapeutics Enterovirus D68
physiological function the enzyme is central for viral genome replication Enterovirus A71
physiological function the enzyme is central for viral genome replication Enterovirus D68