Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.48 extracted from

  • Villalba, B.; Li, J.; Johnson, K.A.
    Resistance to excision determines efficiency of hepatitis C virus RNA-dependent RNA polymerase inhibition by nucleotide analogs (2020), J. Biol. Chem., 295, 10112-10124 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
N-terminal hexa-His-NS5BD21 (Con1 strain, GT1b with the C-terminal 21-amino acid membrane anchor domain deleted), expression in Escherichia coli Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
2'-fluoro-2'C-methylcytidine-5'-triphosphate acts as a chain terminator after incorporated during RNA synthesis. It is readily incorporated into a growing primer by NS5B but is also efficiently excised Hepacivirus C
2'-fluoro-2'C-methyluridine-5'-triphosphate acts as a chain terminator after incorporated during RNA synthesis. It is readily incorporated into a growing primer by NS5B but is also efficiently excised. Although 2'C-modified CTP analogs are more efficiently incorporated compared with the UTP analog, the UTP analog is a better inhibitor because of its resistance to ATP-dependent excision Hepacivirus C
2'C-methylcytidine triphosphate acts as a chain terminator after incorporated during RNA synthesis. It is readily incorporated into a growing primer by NS5B but is also efficiently excised Hepacivirus C
4'-azido-cytidine triphosphate acts as a chain terminator after incorporated during RNA synthesis. It is readily incorporated into a growing primer by NS5B but is also efficiently excised Hepacivirus C
sofosbuvir the clinical effectiveness of sofosbuvir is largely a function of being intractable to nucleotide-mediated excision compared with similar nucleoside analogs Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hepacivirus C

Synonyms

Synonyms Comment Organism
NS5B
-
 Hepacivirus C
RNA-dependent RNA polymerase
-
 Hepacivirus C

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the replication of the hepatitis C virus genome Hepacivirus C