Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.48 extracted from

  • Balakrishnan, A.; Price, E.; Luu, C.; Shaul, J.; Wartchow, C.; Cantwell, J.; Vo, T.; DiDonato, M.; Spraggon, G.; Hekmat-Nejad, M.
    Biochemical characterization of respiratory syncytial virus RNA-dependent RNA polymerase complex (2020), ACS Infect. Dis., 6, 2800-2811 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-protein the intact L protein is essential for enzyme (RdRP) activity and optimal RNA binding. In truncated L protein constructs, enzyme activity is abrogated due to their deficiency in RNA template binding Respiratory syncytial virus type A

Protein Variants

Protein Variants Comment Organism
N812A active site variant of RSV L-protein in the RSV L/P complex. The RSV L(N812A)/P complex does not produce RNA products with TrC sequence RNA as template Respiratory syncytial virus type A

Inhibitors

Inhibitors Comment Organism Structure
additional information not sensitive to actinomycin D Respiratory syncytial virus type A

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00018
-
CTP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
0.0011
-
RNAn 30°C, pH 7.5, RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
0.0014
-
UTP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
0.0027
-
GTP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
0.0085
-
ATP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Respiratory syncytial virus type A the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome diphosphate + RNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Respiratory syncytial virus type A X5F520
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
?
CTP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
?
GTP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome Respiratory syncytial virus type A diphosphate + RNAn+1
-
?
UTP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
More the core polymerase in respiratory syncytial virus (RSV) is composed of two proteins, a 250 kDa polymerase subunit (L), which contains the RNA-dependent RNA polymerase, the polyribonucleotidyl transferase (PRNTase, capping), and the methyltransferase enzymatic domains essential for viral transcription and replication, and the 27 kDa phosphoprotein (P) accessory protein Respiratory syncytial virus type A

Synonyms

Synonyms Comment Organism
L/P RdRP
-
Respiratory syncytial virus type A
RNA-dependent RNA polymerase
-
Respiratory syncytial virus type A

General Information

General Information Comment Organism
physiological function the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome Respiratory syncytial virus type A