Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-protein | the intact L protein is essential for enzyme (RdRP) activity and optimal RNA binding. In truncated L protein constructs, enzyme activity is abrogated due to their deficiency in RNA template binding | Respiratory syncytial virus type A |
Protein Variants | Comment | Organism |
---|---|---|
N812A | active site variant of RSV L-protein in the RSV L/P complex. The RSV L(N812A)/P complex does not produce RNA products with TrC sequence RNA as template | Respiratory syncytial virus type A |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not sensitive to actinomycin D | Respiratory syncytial virus type A |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
CTP | 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | |
0.0011 | - |
RNAn | 30°C, pH 7.5, RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | |
0.0014 | - |
UTP | 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | |
0.0027 | - |
GTP | 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | |
0.0085 | - |
ATP | 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | Respiratory syncytial virus type A | the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome | diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Respiratory syncytial virus type A | X5F520 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNAn | RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | diphosphate + RNAn+1 | - |
? | |
CTP + RNAn | RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | diphosphate + RNAn+1 | - |
? | |
GTP + RNAn | RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome | Respiratory syncytial virus type A | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence | Respiratory syncytial virus type A | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the core polymerase in respiratory syncytial virus (RSV) is composed of two proteins, a 250 kDa polymerase subunit (L), which contains the RNA-dependent RNA polymerase, the polyribonucleotidyl transferase (PRNTase, capping), and the methyltransferase enzymatic domains essential for viral transcription and replication, and the 27 kDa phosphoprotein (P) accessory protein | Respiratory syncytial virus type A |
Synonyms | Comment | Organism |
---|---|---|
L/P RdRP | - |
Respiratory syncytial virus type A |
RNA-dependent RNA polymerase | - |
Respiratory syncytial virus type A |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome | Respiratory syncytial virus type A |