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Literature summary for 2.7.7.48 extracted from
- Purification and biochemical characterisation of rabbit calicivirus RNA-dependent RNA polymerases and identification of non-nucleoside inhibitors (2016), Viruses, 8, 100.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the virus causes significant economic losses in rabbit farming and reduces wild rabbit populations, and its RNA-dependent RNA polymerase (RdRp) is a primary target for the development of antiviral drugs | Rabbit hemorrhagic disease virus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli | Rabbit hemorrhagic disease virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (4E)-1-(3,4-dimethylphenyl)-4-(hydroxyimino)pyrazolidine-3,5-dione | - |
Rabbit hemorrhagic disease virus |  |
| 5-phenyl-2H-1,2,3-triazole-4-carboxylic acid | - |
Rabbit hemorrhagic disease virus | |
| EDTA | - |
Rabbit hemorrhagic disease virus | |
| N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-methyl-2-phenyl-1,3-thiazole-5-carboxamide | a non-nucleoside inhibitor | Rabbit hemorrhagic disease virus | |
| rGTP | - |
Rabbit hemorrhagic disease virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic analysis | Rabbit hemorrhagic disease virus | |
| 14.6 | - |
rGTP | Michaelis-Menten kinetic model, pH 7.5, 30°C, recombinant enzyme | Rabbit hemorrhagic disease virus | |
| 19.1 | - |
rGTP | substrate inhibition, pH 7.5, 30°C, recombinant enzyme | Rabbit hemorrhagic disease virus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rabbit hemorrhagic disease virus | RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity | ? | - |
? | |
| additional information | Rabbit hemorrhagic disease virus V-351 | RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity | ? | - |
? | |
| nucleoside triphosphate + RNAn | Rabbit hemorrhagic disease virus | - |
diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | Rabbit hemorrhagic disease virus V-351 | - |
diphosphate + RNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rabbit hemorrhagic disease virus | P27411 | polyprotein; a calicivirus | - |
| Rabbit hemorrhagic disease virus V-351 | P27411 | polyprotein; a calicivirus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally HIs6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Rabbit hemorrhagic disease virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the in vitro polymerase activity of a non-pathogenic calicivirus RdRp is at least two times higher than that of the RdRp of the highly virulent RHDV | Rabbit hemorrhagic disease virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity | Rabbit hemorrhagic disease virus | ? | - |
? | |
| additional information | RHDV polyprotein cleavage product p58 is an active RNA-dependent RNA polymerase, that has no DNA-dependent RNA polymerase activity | Rabbit hemorrhagic disease virus V-351 | ? | - |
? | |
| nucleoside triphosphate + RNAn | - |
Rabbit hemorrhagic disease virus | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | - |
Rabbit hemorrhagic disease virus V-351 | diphosphate + RNAn+1 | - |
? | |
| rGTP + RNAn | - |
Rabbit hemorrhagic disease virus | diphosphate + RNAn+1 | - |
? | |
| rGTP + RNAn | - |
Rabbit hemorrhagic disease virus V-351 | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | crystal structure of RHDV RdRp reveals that the enzyme adopts a shape that resembles a right hand, with domains corresponding to the fingers, palm and thumb | Rabbit hemorrhagic disease virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RDRP | - |
Rabbit hemorrhagic disease virus |
| RNA-dependent RNA polymerase | - |
Rabbit hemorrhagic disease virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Rabbit hemorrhagic disease virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rabbit hemorrhagic disease virus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
rGTP | substrate inhibition, pH 7.5, 30°C, recombinant enzyme | Rabbit hemorrhagic disease virus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0199 | - |
pH 7.5, 30°C, recombinant enzyme | Rabbit hemorrhagic disease virus | N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-methyl-2-phenyl-1,3-thiazole-5-carboxamide | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the in vitro polymerase activity of a non-pathogenic calicivirus RdRp is at least two times higher than that of the RdRp of the highly virulent RHDV | Rabbit hemorrhagic disease virus |
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