Activating Compound | Comment | Organism | Structure |
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additional information | The Nicotiana benthamiana canonical 9-zinc finger (ZF) transcription factor IIIA (TFIIIA-9ZF) as well as its variant TFIIIA-7ZF both interact with (+)-PSTVd, but only TFIIIA-7ZF interacts with (-)-PSTVd. TFIIIA-7ZF is found in the nucleoplasm and nucleolus, in contrast to the strictly nucleolar localization of TFIIIA-9ZF. Overexpression of TFIIIA-7ZF enhances PSTVd replication in planta, the land plant-specific transcription factor directly enhances transcription of a pathogenic noncoding RNA template by DNA-dependent RNA polymerase II. TFIIIA-7ZF is a cellular transcription factor that acts in DdRP-catalyzed RNA-templated transcription, highlighting both the extraordinary evolutionary adaptation of viroids and the potential of DdRPs for a broader role in cellular processes. Subcellular localization of TFIIIA-7ZF and TFIIIA-9ZF in Nicotiana benthamiana leaves infected by PSTVd, overview | Potato spindle tuber viroid |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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additional information | Potato spindle tuber viroid | Potato spindle tuber viroid replication in the nucleoplasm generates (-)-PSTVd intermediates and (+)-PSTVd copies. The Nicotiana benthamiana canonical 9-zinc finger (ZF) transcription factor IIIA (TFIIIA-9ZF) as well as its variant TFIIIA-7ZF both interact with (+)-PSTVd, but only TFIIIA-7ZF interacts with (-)-PSTVd. TFIIIA-7ZF is found in the nucleoplasm and nucleolus, in contrast to the strictly nucleolar localization of TFIIIA-9ZF. The DNA-dependent RNA polymerase II interacts with the (+)- and (2)-PSTVd RNA in vivo | ? | - |
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Organism | UniProt | Comment | Textmining |
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Potato spindle tuber viroid | - |
Potato spindle tuber viroid (PSTVd) RNA genome in Solanum lycopersicum | - |
Source Tissue | Comment | Organism | Textmining |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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additional information | Potato spindle tuber viroid replication in the nucleoplasm generates (-)-PSTVd intermediates and (+)-PSTVd copies. The Nicotiana benthamiana canonical 9-zinc finger (ZF) transcription factor IIIA (TFIIIA-9ZF) as well as its variant TFIIIA-7ZF both interact with (+)-PSTVd, but only TFIIIA-7ZF interacts with (-)-PSTVd. TFIIIA-7ZF is found in the nucleoplasm and nucleolus, in contrast to the strictly nucleolar localization of TFIIIA-9ZF. The DNA-dependent RNA polymerase II interacts with the (+)- and (2)-PSTVd RNA in vivo | Potato spindle tuber viroid | ? | - |
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General Information | Comment | Organism |
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malfunction | suppression of Nicotiana benthamiana canonical 9-zinc finger transcription factor TFIIIA-7ZF reduces PSTVd replication, and overexpression of TFIIIA-7ZF enhances PSTVd replication in planta | Potato spindle tuber viroid |
physiological function | the DNA-dependent RNA polymerase (DdRP) from Potato spindle tuber viroid (PSTVd) RNA genome in Solanum lycopersicum possesses RNA-dependent RNA polymerase activity. A land plant-specific transcription factor directly enhances transcription of a pathogenic noncoding RNA template by DNA-dependent RNA polymerase II | Potato spindle tuber viroid |