Cloned (Comment) | Organism |
---|---|
expression of wild-type and muztant enzymes in Escherichia coli strain BL21(DE3) | Cystovirus phi6 |
Protein Variants | Comment | Organism |
---|---|---|
E139Q | site-directed mutagenesis in the plough region | Cystovirus phi6 |
E165A | site-directed mutagenesis in the plough region | Cystovirus phi6 |
E165Q | site-directed mutagenesis in the plough region | Cystovirus phi6 |
E168Q | site-directed mutagenesis in the plough region | Cystovirus phi6 |
E172Q | site-directed mutagenesis in the plough region | Cystovirus phi6 |
E634Q | site-directed mutagenesis in the specificity pocket, the mutation to glutamine E634 eliminates this salt bridge without major disruption of the internal packaging within the polymerase | Cystovirus phi6 |
E652Q | site-directed mutagenesis in the plough region | Cystovirus phi6 |
K147L | site-directed mutagenesis in the rim of the template tunnel | Cystovirus phi6 |
K451A | site-directed mutagenesis in the specificity pocket | Cystovirus phi6 |
K451L | site-directed mutagenesis in the specificity pocket | Cystovirus phi6 |
K541A | site-directed mutagenesis in the rim of the template tunnel, the mutation reduces both the positive charge as well as the size and shape of the template tunnel rim | Cystovirus phi6 |
K541L | site-directed mutagenesis in the rim of the template tunnel, the mutation reduces both the positive charge as well as the size and shape of the template tunnel rim | Cystovirus phi6 |
K627A | site-directed mutagenesis in the rim of the template tunnel | Cystovirus phi6 |
K627L | site-directed mutagenesis in the rim of the template tunnel | Cystovirus phi6 |
L628S | site-directed mutagenesis in the specificity pocket | Cystovirus phi6 |
additional information | structure-based mutagenesis of the enzyme, the elongation rates are not compromised by the mutations, while the transcription rates vary, phenotypes, overview | Cystovirus phi6 |
R146L | site-directed mutagenesis in the rim of the template tunnel | Cystovirus phi6 |
R291K | site-directed mutagenesis in the specificity pocket | Cystovirus phi6 |
R291S | site-directed mutagenesis in the specificity pocket | Cystovirus phi6 |
R30A | site-directed mutagenesis in the rim of the template tunnel, the mutation reduces both the positive charge as well as the size and shape of the template tunnel rim | Cystovirus phi6 |
T633A | site-directed mutagenesis in the specificity pocket | Cystovirus phi6 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | Cystovirus phi6 | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cystovirus phi6 | - |
a dsRNA bacteriophage of the family Cystoviridae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | In silico template RNA modelling, overview | Cystovirus phi6 | ? | - |
? | |
nucleoside triphosphate + RNAn | - |
Cystovirus phi6 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | NTPs are ATP, GTP, CTP, and UTP, roles of the negatively charged plough area on the polymerase surface, of the rim of the template tunnel and of the template specificity pocket that is key in the formation of the productive RNA-polymerase binary complex. The positively charged rim of the template tunnel has a significant role in the engagement of highly structured ssRNA molecules, whereas specific interactions further down in the template tunnel promote ssRNA entry to the catalytic site | Cystovirus phi6 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure-function analysis, molecular mapping, overview | Cystovirus phi6 |
Synonyms | Comment | Organism |
---|---|---|
RDRP | - |
Cystovirus phi6 |
RNA-dependent RNA polymerase | - |
Cystovirus phi6 |