Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.48 extracted from

  • Huet, S.; Avilov, S.V.; Ferbitz, L.; Daigle, N.; Cusack, S.; Ellenberg, J.
    Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy (2010), J. Virol., 84, 1254-1264.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
RanBP5 a member of importin beta family, required for PB1/PA heterodimer import into the nucleus, where the active heterotrimer of the enzyme is formed influenza A virus

Protein Variants

Protein Variants Comment Organism
additional information impairing the nuclear import of PB2 by mutating its nuclear localization signal leads to abnormal formation of the trimeric polymerase in the cytoplasm influenza A virus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus efficient polymerase assembly is a limiting factor in the viability of reassortant viruses, mechanism of nuclear import and assembly of the three polymerase subunits, PB1, PB2, and PA, overview. PB1 and PA form a dimer in the cytoplasm, which is imported into the nucleus, separately from PB2. The PB1/PA heterodimer is imported into the nucleus by RanBP5, a member of importin beta family. Once in the nucleus, the PB1/PA dimer associates with PB2 to form the trimeric polymerase influenza A virus 5634
-

Organism

Organism UniProt Comment Textmining
influenza A virus
-
-
-

Subunits

Subunits Comment Organism
trimer mechanism of nuclear import and assembly of the three polymerase subunits, PB1, PB2, and PA, overview. PB1 and PA form a dimer in the cytoplasm, which is imported into the nucleus separately from PB2. Once in the nucleus, the PB1/PA dimer associates with PB2 to form the trimeric polymerase. The strong interface that can be formed between the N-terminal extremity of PB2 and the C-terminal extremity of PB1 is present only in the fully assembled trimeric polymerase influenza A virus

Synonyms

Synonyms Comment Organism
RNA-dependent RNA polymerase
-
influenza A virus

General Information

General Information Comment Organism
malfunction impairing the nuclear import of PB2 by mutating its nuclear localization signal leads to abnormal formation of the trimeric polymerase in the cytoplasm influenza A virus
additional information efficient polymerase assembly is a limiting factor in the viability of reassortant viruses, mechanism of nuclear import and assembly of the three polymerase subunits, PB1, PB2, and PA, overview influenza A virus