Literature summary for 2.7.7.48 extracted from

Maliogka, V.I.; Dovas, C.I.; Lotos, L.; Efthimiou, K.; Katis, N.I.
Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus (2009), Arch. Virol., 154, 209-218.

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Cloned(Commentary)

Cloned (Comment)	Organism
RdRp_2, genome organization analysis, and nucleotide and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His-tagged protein in Escherichia coli strain M15	Grapevine leafroll-associated virus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58200	-	x * 58200, about, sequence calculation	Grapevine leafroll-associated virus

Organism

Organism	UniProt	Comment	Textmining
Grapevine leafroll-associated virus	-	i.e. GLRaV-Pr, an isolate from Greek grapevines, Vitis vinifera cv. Mantilaria, belongs to the Ampelovirus group	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged RdRp_2 from Escherichia coli strain M15 by nickel affiniity chromatography	Grapevine leafroll-associated virus

Subunits

Subunits	Comment	Organism
?	x * 58200, about, sequence calculation	Grapevine leafroll-associated virus
More	the enzyme is part, as RdRp_2 domain, of a 253-kDa polyprotein that also contains a papain-like protease, a methyltransferase, AlkB and helicase domains, a 5.2-kDa hydrophobic protein, a 58.5-kDa heat shock 70 protein homologue, a 60-kDa protein, a 30-kDa coat protein, and a 23-kDa protein	Grapevine leafroll-associated virus

Synonyms

Synonyms	Comment	Organism
RNA-dependent RNA polymerase	-	Grapevine leafroll-associated virus

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Release 2023.1 (January 2023)