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Literature summary for 2.7.7.48 extracted from

  • Yap, T.L.; Chen, Y.L.; Xu, T.; Wen, D.; Vasudevan, S.G.; Lescar, J.
    A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution (2007), Acta Crystallogr. Sect. F, F63, 78-83.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli coli BL21 (DE3) cells Dengue virus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, with 0.1 M Tris-HCl pH 8.5, 0.8 M potassium/sodium tartrate, and 0.5% (w/v) PEG monomethyl ether 5000 Dengue virus

Organism

Organism UniProt Comment Textmining
Dengue virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography, Source 15S column chromatography, and gel filtration Dengue virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
Dengue virus diphosphate + RNAn+1
-
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Synonyms

Synonyms Comment Organism
DENV 3 polymerase
-
Dengue virus
DENV RdRp
-
Dengue virus
NS5
-
Dengue virus
RNA-dependent RNA polymerase
-
Dengue virus