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Literature summary for 2.7.7.48 extracted from
- Crystal structure of the Dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-Angstrom resolution (2007), J. Virol., 81, 4753-4765.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Dengue virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method, using 0.1 M Tris-HCl at pH 8.5, 0.8 M potassium-sodium tartrate, 0.5% (w/v) PEG monomethyl ether 5000 | Dengue virus |
| hanging-drop vapor diffusion method at 4°C, three-dimensional structure of a catalytically active DENV RdRp domain determined to 1.85 A resolution by X-ray crystallography | Dengue virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-dGTP | - |
Dengue virus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn | structure reveals the presence of two zinc ion binding motifs | Dengue virus | |
| Zn2+ | contains zinc, Zn2+ likely contributes to the structural stability of the region near motif E of the DENV 3 polymerase | Dengue virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 104000 | - |
gel filtration | Dengue virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | Dengue virus | - |
diphosphate + RNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dengue virus | - |
- |
- |
| Dengue virus | Q6DLV0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap HP column chromatography, 15S column chromatography, ultrafiltration, and Superdex 200 gel filtration | Dengue virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | - |
Dengue virus | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | homopolymer C as the template and biotinoligo(G)20 as the primer | Dengue virus | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | using homopolymer C as the template | Dengue virus | diphosphate + RNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DENV 3 RdRp | - |
Dengue virus |
| DENV RdRp | - |
Dengue virus |
| NS5 protein | - |
Dengue virus |
| NS5 protein | bifunctional and contains 900 amino acids. The S-adenosyl methionine transferase activity resides within its N-terminal domain, and residues 270 to 900 form the RNA-dependent RNA polymerase (RdRp) catalytic domain | Dengue virus |
| RNA-dependent RNA polymerase | - |
Dengue virus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00022 | - |
- |
Dengue virus | 3'-dGTP | |
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