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Literature summary for 2.7.7.48 extracted from
- Structural and functional characterization of sapovirus RNA-dependent RNA polymerase (2007), J. Virol., 81, 1858-1871.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fusion protein bearing a His6 tag at its C-terminus or at its N terminus is overexpressed in Escherichia coli | Sapovirus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| overall structure of the sapovirus 3Dpol is determined by X-ray crystallography to 2.32 A resolution | Sapovirus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ | Sapovirus |  |
| Mn2+ | over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ | Sapovirus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, SDS-PAGE | Sapovirus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | Sapovirus | key enzyme of replication | diphosphate + RNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sapovirus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | key enzyme of replication | Sapovirus | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | in a synthetic RNA template-dependent reaction, sapovirus 3Dpol synthesizes a double-stranded RNA or labels the template 3' terminus by terminal transferase activity. Initiation of RNA synthesis occurs de novo on heteropolymeric templates or in a primer dependent manner on polyadenylated templates | Sapovirus | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, SDS-PAGE | Sapovirus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3Dpol-like protein | the RNA-dependent RNA polymerase is associated with the 3Dpol-like protein | Sapovirus |
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Release 2023.1 (January 2023)
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