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Literature summary for 2.7.7.48 extracted from

  • Kim, Y.G.; Yoo, J.S.; Kim, J.H.; Kim, C.M.; Oh, J.W.
    Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymerase (2007), BMC Mol. Biol., 8, 59.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus is expressed in Escherichia coli Japanese encephalitis virus
expressed in Escherichia coli Japanese encephalitis virus

Protein Variants

Protein Variants Comment Organism
D668A inactive Japanese encephalitis virus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ K+ exhibits a stimulatory effect at 25 mM, while the stimulatory effect decreases when the concentration of K+ is higher than 25 mM Japanese encephalitis virus
Mn2+ dependent on Mn2+, optimal concentration at 2.5 mM Japanese encephalitis virus
additional information no detectable level of RdRp activity is observed in the presence of 0.5 to 10 mM Mg2+ Japanese encephalitis virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
SDS-PAGE Japanese encephalitis virus

Organism

Organism UniProt Comment Textmining
Japanese encephalitis virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus Japanese encephalitis virus
SP-Sepharose column chromatography Japanese encephalitis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn the NS5 protein is able to use both plus- and minus-strand 3'-untranslated regions of the JEV genome as templates in the absence of a primer, with the latter RNA being a better template. Analysis of the RNA synthesis initiation site using the 3'-end 83 nucleotides of the JEV genome as a minimal RNA template reveals that the NS5 protein specifically initiates RNA synthesis from an internal site, U81, at the two nucleotides upstream of the 3'-end of the template Japanese encephalitis virus diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn poly(A) RNA template Japanese encephalitis virus diphosphate + RNAn+1
-
?

Synonyms

Synonyms Comment Organism
JEV NS5 nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains Japanese encephalitis virus
JEV RdRp
-
Japanese encephalitis virus
NS5 RdRp NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase domains, it is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins Japanese encephalitis virus
RNA-dependent RNA polymerase
-
Japanese encephalitis virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Japanese encephalitis virus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
rapid decrease of activity above pH 8 Japanese encephalitis virus