Cloned (Comment) | Organism |
---|---|
enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus is expressed in Escherichia coli | Japanese encephalitis virus |
expressed in Escherichia coli | Japanese encephalitis virus |
Protein Variants | Comment | Organism |
---|---|---|
D668A | inactive | Japanese encephalitis virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | K+ exhibits a stimulatory effect at 25 mM, while the stimulatory effect decreases when the concentration of K+ is higher than 25 mM | Japanese encephalitis virus | |
Mn2+ | dependent on Mn2+, optimal concentration at 2.5 mM | Japanese encephalitis virus | |
additional information | no detectable level of RdRp activity is observed in the presence of 0.5 to 10 mM Mg2+ | Japanese encephalitis virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
SDS-PAGE | Japanese encephalitis virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Japanese encephalitis virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus | Japanese encephalitis virus |
SP-Sepharose column chromatography | Japanese encephalitis virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | the NS5 protein is able to use both plus- and minus-strand 3'-untranslated regions of the JEV genome as templates in the absence of a primer, with the latter RNA being a better template. Analysis of the RNA synthesis initiation site using the 3'-end 83 nucleotides of the JEV genome as a minimal RNA template reveals that the NS5 protein specifically initiates RNA synthesis from an internal site, U81, at the two nucleotides upstream of the 3'-end of the template | Japanese encephalitis virus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | poly(A) RNA template | Japanese encephalitis virus | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
JEV NS5 | nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains | Japanese encephalitis virus |
JEV RdRp | - |
Japanese encephalitis virus |
NS5 RdRp | NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase domains, it is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins | Japanese encephalitis virus |
RNA-dependent RNA polymerase | - |
Japanese encephalitis virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Japanese encephalitis virus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
rapid decrease of activity above pH 8 | Japanese encephalitis virus |