KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
CTP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.0006 | - |
UTP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.0014 | - |
GTP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.0029 | - |
ATP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.0057 | - |
UTP | pH 7.5, 23°C, C-terminally truncated enzyme form NS5B(DELTA21), reaction with dT16/poly(rA) | Hepacivirus C | |
0.013 | - |
UTP | pH 7.5, 23°C, full-length enzyme, reaction with dT16/poly(rA) | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
C-terminally truncated enzyme form NS5B(DELTA21) expressed in Escherichia coli | Hepacivirus C |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.025 | - |
truncated enzyme form NS5B(DELTA21) | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
CTP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
GTP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | once synthesis has begun, the C-terminally truncated enzyme NS5B(DELTA21) does not dissociate from the template until a complete double strand copy of the RNA is made | Hepacivirus C | diphosphate + RNAn+1 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000105 | - |
ATP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.000133 | - |
CTP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.000167 | - |
UTP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.000383 | - |
GTP | pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) | Hepacivirus C | |
0.00167 | - |
UTP | pH 7.5, 23°C, full-length enzyme, reaction with dT16/poly(rA) | Hepacivirus C | |
0.0272 | - |
UTP | pH 7.5, 23°C, C-terminally truncated enzyme form NS5B(DELTA21), reaction with dT16/poly(rA) | Hepacivirus C |