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Literature summary for 2.7.7.48 extracted from

  • Alaoui-Lsmaili, M.H.; Hamel, M.; L'Heureux, L.; Nicolas, O.; Bilimoria, D.; Labonte, P.; Mounir, S.; Rando, R.F.
    The Hepatitis C virus NS5B RNA-dependent RNA polymerase activity and susceptibility to inhibitors is modulated by metal cations (2000), J. Hum. Virol., 3, 306-316.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant full-length enzyme is expressed in insect cells Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
2',3'-dideoxycytidine triphosphate inhibition is higher in presence of Mn2+ than in presence of Mg2+ Hepacivirus C
2',3'-dideoxyguanosine triphosphate inhibition is higher in presence of Mn2+ than in presence of Mg2+ Hepacivirus C
2'-deoxycytidine triphosphate inhibition is higher in presence of Mn2+ than in presence of Mg2+ Hepacivirus C
2'-dGTP inhibition is higher in presence of Mn2+ than in presence of Mg2+ Hepacivirus C
gliotoxin in presence of MnCl2 the IC50 value is 0.036 mM, in presence of Mn2+ the IC50 value is 0.202 mM Hepacivirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of Mg2+ and Mn2+ on the Km-value for polyC/oligoG and polyA/oligodT Hepacivirus C

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal polymerase activity at 10 mM MgCl2 Hepacivirus C
Mn2+ optimal polymerase activity at 0.25 mM MnCl2, activity is lower than in presence of MgCl2 Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length enzyme expressed in baculovirus Sf9 insect cells Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn polyC/oligoG is more efficient in supporting the HCV NS5B polymerase activity than polyA/oligodT. PolyA/oligoU or polyI/oligodC Hepacivirus C diphosphate + RNAn+1
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.202
-
 in presence of MnCl2 the IC50 value is 0.036 mM, in presence of Mn2+ the IC50 value is 0.202 mM Hepacivirus C gliotoxin