Literature summary for 2.7.7.48 extracted from

Ferrari, E.; Wright-Minogue, J.; Fang, J.W.; Baroudy, B.M.; Lau, J.Y.; Hong, Z.
Characterization of soluble Hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli (1999), J. Virol., 73, 1649-1654.

Search for more literature for 2.7.7.48

Inhibitors

Inhibitors	Comment	Organism	Structure
gliotoxin	-	Hepacivirus C
Zn2+	-	Hepacivirus C

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.052	-	GTP	poly(C)-oligo(G) as template	Hepacivirus C

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	to improve the solubility of NS5B, the hydrophobic C-terminus containing 21 amino acids is removed, yielding a truncated NS5B which is highly soluble and monodispersed in the absence of detergents	Hepacivirus C	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	for optimal activity Mn2+ is the preferred divalent cation	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	expressed in Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + RNAn	-	Hepacivirus C	diphosphate + RNAn+1	-	?
nucleoside triphosphate + RNAn	-	Hepacivirus C	diphosphate + RNAn+1	-	?

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
22	37	when the assay temperature is increased from 22°C to 37°C the turnover number is significantly increased	Hepacivirus C

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.005	-	GTP	poly(C)-oligo(G) as template	Hepacivirus C

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.23	-	gliotoxin	-	Hepacivirus C

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Release 2023.1 (January 2023)